Ulcer healing effect of autologous mixed sheets consisting of fibroblasts and peripheral blood mononuclear cells in rabbit ischemic hind limb.
Ulcer healing effect of autologous mixed sheets consisting of fibroblasts and peripheral blood mononuclear cells in rabbit ischemic hind limb.
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发表时间：
2017
影响因子：
2.2
通讯作者：
Y. Takeuchi;Koji Ueno;T. Mizoguchi;M. Samura;Takasuke Harada;A. Oga;Tomoaki Murata;Tohru Hosoyama;N. Morikage;K. Hamano
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作者：
Y. Takeuchi;Koji Ueno;T. Mizoguchi;M. Samura;Takasuke Harada;A. Oga;Tomoaki Murata;Tohru Hosoyama;N. Morikage;K. Hamano
我们开发了由成纤维细胞和外周血单核细胞组成的混合细胞片，这些细胞片具有高效分泌血管内皮生长因子的能力。本研究的目的是证实混合片对兔后肢缺血溃疡的治疗效果。我们采用植入并将皮肤夹在两块磁铁之间构建的溃疡模型作为人类难治性皮肤溃疡的代表。在愈合初期，混合细胞片的溃疡愈合率高于对照组。小牛血压和血管造影评分被认为反映粗略的侧支血流，在混合细胞片中没有变化。然而，通过激光多普勒灌注成像，混合细胞片的植入显示，与重组人碱性成纤维细胞生长因子小黄蛋白和对照组相比，缺血肢体愈合皮肤的微血管血流明显改善。这些结果提示，混合细胞可能主要通过其血管生成能力作用于缺血组织表面，从而促进缺血性溃疡的愈合。混合细胞片有望成为难治性皮肤溃疡的治疗材料。
We developed mixed cell sheets consisting of fibroblasts and peripheral blood mononuclear cells that had high potency for secreting vascular endothelial growth factor. The purpose of this study was to confirm the therapeutic effects of mixed sheets in rabbits suffering from ulcers at the ischemic hind limbs. We used the ulcer model, which was constructed by implantation and sandwiching the skin between two magnets to be a representative of human refractory cutaneous ulcer. The ulcer healing rate of mixed cell sheets was higher than that of the control at an early stage of healing. The calf blood pressure and angiographic score, which were considered to reflect rough collateral blood flow, did not vary amongmixed cell sheets. However, through laser Doppler perfusion image, implantation of mixed cell sheets revealed a significant improvement in microvascular blood flow in the healed skin of the ischemic limb compared to trafermin, a recombinant human basic fibroblast growth factor, and the control. These results suggest that mixed cell may operate predominantly on the surface of the ischemic tissue by their angiogenic potency, thereby promoting healing of the ischemic ulcer. Mixed cell sheets could become a promising therapeutic material for refractory cutaneous ulcers.