ELASTASE, COLLAGENASE, AND THE BIAXIAL ELASTIC PROPERTIES OF DOG CAROTID-ARTERY
ELASTASE, COLLAGENASE, AND THE BIAXIAL ELASTIC PROPERTIES OF DOG CAROTID-ARTERY
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DOI：
10.1152/ajpheart.1984.247.1.h124
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发表时间：
1984-01-01
影响因子：
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通讯作者：
CANFIELD, TR
中科院分区：
文献类型：
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作者：
DOBRIN, PB;CANFIELD, TR
体外研究犬颈动脉的4个纵伸比。lambda。Z = 1.2, 1.4, 1.6, 1.8，按随机顺序排列。在每段长度上，压力逐步升高到200毫米汞柱或直到血管弯曲。在对照条件下研究血管，并在中等剂量的降解酶处理后：80 U/ml弹性酶90分钟或640 U/ml胶原酶120分钟。根据初步研究，选择这些剂量是为了降解血管，而不是破坏血管。弹性蛋白酶处理（n = 24）降低了所有血管长度的纵向和周向应力。当压力大于15 mm Hg时，周向应力减小，且随纵向和周向变形的增大而增大。无论容器长度如何，纵向应力都以恒定的量减小。当血管扩张至少60 mm Hg时，胶原酶处理（n = 24）降低了周向应力；它没有减少纵向应力。在犬颈动脉的完整圆柱形段中，受到生理水平的应变，弹性蛋白显然同时承担圆周和纵向载荷的一部分，而胶原蛋白显然只承担圆周载荷的一部分。
Segments of dog carotid artery were studied in vitro at 4 longitudinal extension ratios, .lambda.z = 1.2, 1.4, 1.6 and 1.8, in randomized order. At each length, the pressure was elevated in steps to 200 mm Hg or until the vessels buckled. Vessels were studied under control conditions and after treatment with moderate doses of degradative enzymes: 80 U/ml elastase for 90 min or 640 U/ml collagenase for 120 min. These doses were selected, following pilot studies, to degrade vessels but not to destroy them. Treatment with elastase (n = 24) reduced both longitudinal and circumferential stresses at all vessel lengths. Circumferential stress was reduced at pressures greater than 15 mm Hg, the magnitude of effect increasing with both longitudinal and circumferential deformations. Longitudinal stress was reduced by a constant amount, irrespective of vessel length. Treatment with collagenase (n = 24) reduced circumferential stress when the vessels were distended by at least 60 mm Hg; it did not reduce longitudinal stress. In intact cylindrical segments of dog carotid artery, subjected to physiological levels of strain, elastin apparently bears a portion of both circumferential and longitudinal loads, whereas collagen apparently bears a portion of only circumferential loads.