Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production

Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production
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DOI:
10.1016/j.biortech.2007.06.045
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发表时间:
2008-05-01
影响因子:
11.4
通讯作者:
Guebitz, G. M.
Guebitz, G. M.
中科院分区:
工程技术1区
文献类型:
--
作者:
Angerbauer, C.;Siebenhofer, M.;Guebitz, G. M.

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研究了在污水污泥上生长的斯塔克氏脂菌积累脂质的潜力。在合成培养基上,脂质的积累强烈依赖于碳氮比。C/ n比为150时,脂质含量最高,占干物质的68%;C/ n比为60时,脂质含量仅为40%。在pH 5.0 ~ 7.5范围内,pH 5.0时脂质积累最高,pH 6.5时每升产量最高。虽然污水污泥对L. starkeyi的生长和积累没有抑制作用,但当添加到合成培养基时,未经处理的污水污泥对L. starkeyi没有明显的生长抑制作用。然而,通过碱性或酸水解、热或超声处理对污泥进行预处理,会导致L. starkeyi积累脂质,超声预处理获得的脂质最大值为1 g L-1。根据游离脂肪酸和磷的含量,污泥中积累的脂质可以作为生产生物柴油的底物。(C) 2007 Elsevier Ltd.版权所有。
The potential of accumulation of lipids by Lipomyces starkeyi when grown on sewage sludge was assessed. On a synthetic medium, accumulation of lipids strongly depended on the C/N ratio. The highest content of lipids was measured at a C/N-ratio of 150 with 68% lipids of the dry matter while at a C/N-ratio of 60 only 40% were accumulated. Within a pH range from 5.0 to 7.5 the highest lipid accumulation was found at pH 5.0 while the highest yield per litre was pH 6.5. Although sewage sludge had no inhibitory effects on growth or accumulation on L. starkeyi, when added to synthetic medium, there was no significant growth on untreated sewage sludge. However, pretreatment of sludge by alkaline or acid hydrolysis, thermal or ultrasonic treatment lead to accumulation of lipids by L. starkeyi with highest values of 1 g L-1 obtained with ultrasound pre-treatment. Based on the content of free fatty acids and phosphorus, lipids accumulated from sewage sludge could serve as a substrate for the production of biodiesel. (C) 2007 Elsevier Ltd. All rights reserved.