Plasma fibronectin enhances phagocytosis of opsonized particles by human peripheral blood monocytes.
Plasma fibronectin enhances phagocytosis of opsonized particles by human peripheral blood monocytes.
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DOI：
10.1084/jem.157.6.1844
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发表时间：
1983-06-01
影响因子：
15.3
通讯作者：
Brown, E J
中科院分区：
文献类型：
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作者：
Pommier, C G;Inada, S;Fries, L F;Takahashi, T;Frank, M M;Brown, E J
本文研究了血浆纤维连接蛋白（Fn）对人外周血单核细胞结合绵羊红细胞（E）和吞噬绵羊红细胞（E）的影响。在Fn存在或不存在的情况下，未调理的E均不被吞噬，但Fn增强了携带IgG的E的吞噬作用。绵羊红细胞致敏IgM和C3b的摄入时，单核细胞暴露于Fn。Fn增强的吞噬作用发生在液相和玻璃粘附单核细胞。在将单核细胞与经调理的E混合之前洗掉Fn的实验表明，Fn效应的发生是因为与单核细胞而不是经调理的颗粒的相互作用。Fn在Biogel A 1.5m上的层析显示，吞噬增强活性与Fn蛋白完全共层析。Fn不增加单核细胞膜受体的单体IgG的Fc片段的数量。我们得出的结论是，Fn增强单核细胞的吞噬作用，不是通过像传统调理素那样与颗粒结合，而是通过刺激单核细胞更贪婪地摄入已经调理的颗粒。
We have investigated the effect of plasma fibronectin (Fn) on binding and phagocytosis of sheep erythrocytes (E) by human peripheral blood monocytes. Unopsonized E were not phagocytosed in the absence or presence of Fn, but Fn enhanced the phagocytosis of E bearing IgG. Sheep erythrocytes sensitized with IgM and C3b were ingested only when monocytes were exposed to Fn. The Fn enhancement of phagocytosis occurred for both fluid-phase and glass-adherent monocytes. Experiments in which Fn was washed out before mixing monocytes with opsonized E demonstrated that the Fn effect occurred because of interaction with the monocytes and not the opsonized particles. Chromatography of the Fn on Biogel A 1.5m showed that the phagocytosis-enhancing activity exactly co-chromatographed with the Fn protein. Fn did not increase the number of monocyte membrane receptors for the Fc fragment of monomeric IgG. We conclude that Fn enhances monocyte phagocytosis, not by binding to particles as a conventional opsonin, but by stimulating monocytes to ingest already opsonized particles more avidly.