Sequence studies of several alphavirus genomic RNAs in the region containing the start of the subgenomic RNA.
Sequence studies of several alphavirus genomic RNAs in the region containing the start of the subgenomic RNA.
复制标题
对包含亚基因组 RNA 起始区域的几种甲病毒基因组 RNA 进行序列研究。
DOI：
10.1073/pnas.79.17.5235
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发表时间：
1982
影响因子：
11.1
通讯作者：
Strauss,JH
中科院分区：
文献类型：
--
作者：
Ou,JH;Rice,CM;Dalgarno,L;Strauss,EG;Strauss,JH
甲型病毒感染后产生两个mRNAs：基因组(49S)RNA和亚基因组(26S)RNA，基因组(49S)RNA翻译成非结构(复制酶)蛋白，亚基因组(26S)RNA作为病毒粒子结构蛋白的mRNA。测定了几种甲型病毒基因组核糖核酸5‘端的序列和基因组核糖核酸的5’侧翼序列。发现了一个高度保守的21个核苷酸序列，它包括亚基因组RNA的前两个核苷酸和它之前的19个核苷酸。我们认为，该序列在负链中的互补部分是病毒转录酶用于启动26S RNA转录的识别位点，通常在RNA病毒中使用这样的短识别序列。每种病毒的非结构多蛋白前体的COOH末端序列都已推导出来。这些蛋白质序列高度同源，每种情况下都有多个同相终止密码子聚集在26S RNA的非翻译区。与所提出的转录酶识别位点相反，在这些病毒的进化过程中，用于给定保守氨基酸的特定三联体发生了显著差异。然而，蛋白质同源性足以推断出RNA的正确编码阶段，并允许在不知道整个基因组序列的情况下对来自不同甲型病毒的相应核酸序列数据进行比对。
The alphaviruses produce two mRNAs after infection: the genomic (49S) RNA which is translated into the nonstructural (replicase) proteins and the subgenomic (26S) RNA which serves as the mRNA for the virion structural proteins. The sequence of the region of the genomic RNA that contains the 5' end of the subgenomic RNA and the 5' flanking sequences in the genomic RNA were determined for several alphaviruses. A highly conserved sequence of 21 nucleotides was found which includes the first two nucleotides of the subgenomic RNA and the 19 nucleotides preceding it. We propose that the complement of this sequence in the minus strand is the recognition site used by the viral transcriptase for initiation of transcription of 26S RNA and that, in general, such short recognition sequences are commonly used among the RNA viruses. The COOH-terminal sequence of the nonstructural polyprotein precursor has been deduced for each virus. These protein sequences are highly homologous and are followed by multiple in-phase termination codons clustered in the nontranslated region of the 26S RNA in each case. In contrast to the proposed transcriptase recognition site, the particular triplets used for a given conserved amino acid have diverged markedly during evolution of these viruses. The protein homology is sufficient, however, for deduction of the correct coding phase of the RNA and allows the alignment of the corresponding nucleic acid sequence data from different alphaviruses without knowledge of the sequence of the entire genomes.