Insensitivity of Lepidopteran tissues to ouabain : absence of ouabain binding and Na^+-K^+ ATPases in larval and adult midgut
Insensitivity of Lepidopteran tissues to ouabain : absence of ouabain binding and Na^+-K^+ ATPases in larval and adult midgut
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鳞翅目组织对哇巴因不敏感：幼虫和成虫中肠中不存在哇巴因结合和 Na^ -K^ ATP 酶
DOI：
10.1016/0022-1910(77)90261-x
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发表时间：
1977
影响因子：
2.2
通讯作者：
G. L. Vaughan
中科院分区：
文献类型：
--
作者：
A. M. Jungreis;G. L. Vaughan
研究了三种植食性鳞翅目昆虫中肠上皮对哇巴因的不敏感性：烟草天蛾、天蛾、天蛾和帝王蝶。在8 mM K-存在的情况下，中肠未能选择性结合哇巴因。不能证实中肠中存在K+刺激的哇巴因敏感的Na+-K+-ATP酶。神经元组织收集自同一物种在相同的发展阶段，哇巴因容易结合，并具有K+刺激哇巴因敏感的Na+-K+-ATP酶。有人建议，碱金属运输通过这些植食性鳞翅目中肠上皮细胞发生能量相关的过程，不需要Na + ATP酶。
Insensitivity of midgut epithelium to ouabain was studied in three phytophyagous Lepidoptera: the tobacco hornworm.Manduca sexta, the Cecropia silkmoth.Hyalophora cecropia, and the Monarch butterfly,Danaus plexippus. The midgut failed to selectively bind ouabain in the presence of 8 mM K−. The presence of K+stimulated ouabain sensitive Na+K+-ATPases in midgut could not be confirmed. Neuronal tissues collected from the same species at the same stages in development bound ouabain readily, and possessed K+stimulated ouabain sensitive Na+K+-ATPases. It is proposed that alkali metal transport across the midgut epithelium of these phytophagous Lepidoptera occurs via energy-linked processes not requiring Na+K+-ATPases.