A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolytica from glycerol
A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolytica from glycerol
复制标题
一种新型渗透压控制补料分批发酵策略，用于改善解脂耶氏酵母从甘油中生产赤藓糖醇。
DOI：
10.1016/j.biortech.2013.10.031
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发表时间：
2014-01-01
影响因子：
11.4
通讯作者：
Lin, Chi-Chung
中科院分区：
文献类型：
--
作者：
Yang, Li-Bo;Zhan, Xiao-Bei;Lin, Chi-Chung
研究了渗透压对以甘油为唯一碳源的解脂耶氏酵母（Yarrowia lipolytica）CICC 1675发酵生产甘露醇和甘露醇的影响。发现适当的高渗透压可促进甘露醇的产生并抑制甘露醇的形成。为了提高发酵产率和生产能力，在动力学分析的基础上，提出了一种新的两级渗透压控制流加策略。在前96 h期间，通过补料甘油将渗透压维持在4.25 μ l/kg，以减少对细胞生长的抑制。132 h后，将渗透压控制在4.94 μ l/kg，以维持较高的dp（ery）/dt。最大产酶量为194.3 g/L，生产效率为0.95 g/L/h，分别为25.7%和2.2%，比一段补料分批发酵的最佳结果有所提高。这是第一次报道，一种新的渗透压控制补料分批策略显着提高了发酵液的生产。(C)2013爱思唯尔有限公司保留所有权利。
The effect of osmotic pressure on erythritol and mannitol production by an osmophilic yeast strain of Yarrowia lipolytica CICC 1675 using glycerol as the sole carbon source was investigated. Appropriately high osmotic pressure was found to enhance erythritol production and inhibit mannitol formation. A novel two-stage osmotic pressure control fed-batch strategy based on the kinetic analysis was developed for higher erythritol yield and productivity. During the first 96 h, the osmotic pressure was maintained at 4.25 osmol/kg by feeding glycerol to reduce the inhibition of cell growth. After 132 h, the osmotic pressure was controlled at 4.94 osmol/kg to maintain a high dp(ery)/dt. Maximum erythritol yield of 194.3 g/L was obtained with 0.95 g/L/h productivity, which were 25.7% and 2.2%, respectively, improvement over the best results in one-stage fed-batch fermentation. This is the first report that a novel osmotic pressure control fed-batch strategy significantly enhanced erythritol production. (C) 2013 Elsevier Ltd. All rights reserved.