Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor.
Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor.
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DOI：
10.1016/j.biortech.2009.10.050
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发表时间：
2010-03
影响因子：
11.4
通讯作者：
S. Ni;Po-Heng Lee;A. Fessehaie;B. Gao;S. Sung
中科院分区：
文献类型：
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作者：
S. Ni;Po-Heng Lee;A. Fessehaie;B. Gao;S. Sung
将无纺布膜组件与厌氧反应器相连接，设计了一种新型的厌氧氨氧化反应器结构。厌氧氨氧化无纺布膜反应器（ANMR）通过在反应器中形成聚集体和在无纺布膜的内表面上形成生物膜来表现出高的生物量保留能力。在成熟生物膜形成后，膜上没有发生污染问题。运行8个月后，系统的氮负荷（NLR）和氮去除率（NRR）分别达到1263 mgN/l/d和1047.5mgN/l/d，最大比耗氨率（SAC）为51 nmol/mg protein/min，稳态时平均氨氮和亚硝酸盐去除率分别为90.9%和95.0%。厌氧氨氧化物聚集体和生物膜的形态观察显示出高度的致密性。此外，通过实时聚合酶链反应（PCR）分析定量的厌氧氨氧化细菌的富集为97.7%。
An innovative reactor configuration for Anammox enrichment by connecting a non-woven membrane module with an anaerobic reactor was developed in this study. The Anammox non-woven membrane reactor (ANMR) exhibited high biomass retention ability through the formation of aggregates in the reactor and biofilm on the interior surface of the non-woven membrane. No fouling problems occurred on the membrane after the development of mature biofilms. After 8months of operation, the nitrogen loading rate (NLR) and nitrogen removal rate (NRR) reached 1263mgN/l/d and 1047.5mgN/l/d, respectively, with a maximum specific ammonium consumption (SAC) of 51nmol/mg protein/min. At steady state, the average ammonium and nitrite removal efficiencies were 90.9% and 95.0%, respectively. Morphological observation of Anammox aggregates and biofilm showed a high degree of compactness. Also, enrichment of Anammox bacteria was quantified by real-time polymerase chain reaction (PCR) analysis as 97.7%.