Microarray analysis of normal and dystrophic skeletal muscle
Microarray analysis of normal and dystrophic skeletal muscle
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DOI：
10.1016/s0736-5748(02)00041-2
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发表时间：
2002-06-01
影响因子：
1.8
通讯作者：
Kunkel, LM
中科院分区：
文献类型：
--
作者：
Haslett, JN;Kunkel, LM
用于全面RNA表达分析的DNA微阵列的发展及其日益普遍的应用，对分子病理学的研究产生了重大影响。DNA微阵列是核酸斑点有序的高密度排列，可作为整体基因表达分析的基质。在其发展之前，技术限制使得生物过程背后的分子机制必须分解为各个组成部分，并且每个基因或蛋白质都要单独研究。这种方法专注于科学现象的一个单一方面，无法认识或理解生物通路并非孤立存在，而是受到众多因素影响这一事实。在过去十年中取得了巨大的技术进步，现在高密度DNA微阵列能够同时快速测量数千种不同的转录本。这些实验带来了令人兴奋的机会，可以研究生物通路的所有复杂性，并将组织病理学研究中推断出的假设与分子病理学的发现进行比较。这篇综述重点关注微阵列技术如何被用于探究肌肉基因表达，特别是营养不良和正常骨骼肌的差异表达分析所产生的数据如何有助于理解肌肉营养不良的分子病理生理学通路。(C) 2002 ISDN。由爱思唯尔科学有限公司出版。保留所有权利。
The development and increasingly common use of DNA microarrays for comprehensive RNA expression analysis has had a substantial impact on the study of molecular pathology. DNA microarrays are orderly, high-density arrangements of nucleic acid spots that can be used as substrates for global gene expression analysis. Prior to their development, technical limitations necessitated that the molecular mechanisms underlying biological processes be broken down into their component parts and each gene or protein studied individually. This approach, focused as it is on a single aspect of a scientific phenomenon, does not allow appreciation or understanding of the fact that biological pathways do not exist in isolation, but are influenced by numerous factors. Enormous technological advances have been made over the past decade and now high-density DNA microarrays can provide rapid measurement of thousands of distinct transcripts simultaneously. These experiments raise the exciting opportunity to examine biological pathways in all their complexity and to compare the hypotheses deduced from the study of histological pathology with the findings of molecular pathology. This review focuses on how microarray technology has been used to interrogate muscular gene expression and, in particular, on how data generated from differential expression analysis of dystrophic and normal skeletal muscle has contributed to understanding the molecular pathophysiological pathways of muscular dystrophy. (C) 2002 ISDN. Published by Elsevier Science Ltd. All rights reserved.