Human Semenogelin 1 Promotes Sperm Survival in the Mouse Female Reproductive Tract
Human Semenogelin 1 Promotes Sperm Survival in the Mouse Female Reproductive Tract
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DOI：
10.3390/ijms21113961
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发表时间：
2020-06-01
影响因子：
5.6
通讯作者：
Kawano, Natsuko
中科院分区：
文献类型：
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作者：
Sakaguchi, Daiki;Miyado, Kenji;Kawano, Natsuko
精原蛋白1(Semenogelin 1，SEMG1)是人类精浆的主要成分，是一种参与调节精子活力和生育能力的多功能蛋白质。SEMG1与小鼠精囊分泌物2(SVS2)同源，SVS2是精子交配后在雌性生殖道存活所必需的；然而，它在体内的功能尚不清楚。在这项研究中，我们通过检测重组SEMG1对小鼠宫内精子存活的影响来解决这个问题。SEMG1对小鼠精子活力的影响与对人精子的影响相似，呈剂量依赖性降低，而SVS2对小鼠精子活力无影响。将100 mU M SEMG1(不影响小鼠精子活力的浓度)的小鼠附睾精子注射到小鼠子宫内(宫内受精，IUI)。IUI联合SEMG1可显著提高小鼠宫内精子存活率。SEMG1对宫内精子存活率的影响与SVS2相当。对于临床应用，三种易于处理的潜在精子保护多肽是从SEMG1设计的，但它们的单独使用无法模仿SEMG1的能力。我们的结果表明，SEMG1在有效的IUI以及安全、简单和有效的内受精方面具有潜在的临床应用价值。
Semenogelin 1 (SEMG1), a main component of human seminal plasma, is a multi-functional protein involved in the regulation of sperm motility and fertility. SEMG1 is orthologous to mouse seminal vesicle secretion 2 (SVS2), required for sperm survival in the female reproductive tract after copulation; however, its in vivo function remains unclear. In this study, we addressed this issue by examining the effect of recombinant SEMG1 on intrauterine mouse sperm survival. SEMG1 caused a dose-dependent decrease in mouse sperm motility, similar to its effect on human sperm, but SVS2 had no effect on mouse sperm motility. Mouse epididymal sperm in the presence of 100 mu M SEMG1, a concentration that does not affect mouse sperm motility, were injected into the mouse uterus (intrauterine insemination, IUI). IUI combined with SEMG1 significantly increased the survival rate of intrauterine mouse sperm. The effect of SEMG1 on intrauterine sperm survival was comparable with that of SVS2. For clinical applications, three potentially sperm-protecting polypeptides that are easy to handle were designed from SEMG1, but their individual use was unable to mimic the ability of SEMG1. Our results indicate that SEMG1 has potential clinical applications for effective IUI and thereby for safe, simple, and effective internal fertilization.