HUMAN SEMINAL ALPHA-INHIBINS - ISOLATION, CHARACTERIZATION, AND STRUCTURE
HUMAN SEMINAL ALPHA-INHIBINS - ISOLATION, CHARACTERIZATION, AND STRUCTURE
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DOI：
10.1073/pnas.82.12.4041
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
CHUNG, D
中科院分区：
文献类型：
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作者：
LI, CH;HAMMONDS, RG;CHUNG, D
另外两个具有抑制素样活性的多肽已经从人的精浆中分离出来。一个由52个氨基酸组成，另一个由92个氨基酸组成。它们被命名为α-抑制素-52和α-抑制素-92。序列分析表明，α-抑制素-52的NH2端31个氨基酸与以前报道的抑制素样多肽[ILP-(1-31)，现命名为α-抑制素-31]的结构相同，而α-抑制素-92的COOH-端52个氨基酸与α-抑制素-52的结构相同。α-抑制素-92的氨基酸序列为：(序列见正文)小鼠脑垂体体外生物测定数据表明，α-抑制素-52的活性是α-抑制素-31的3.4倍，而α-抑制素-92的活性是α-抑制素-31的40倍以上。放射免疫分析数据表明，α-抑制素-52和α-抑制素-92只有60%的免疫反应性。
Two additional peptides with inhibin-like activity have been isolated from human seminal plasma. One consists of 52 amino acids and the other, 92 amino acids. They are designated alpha-inhibin-52 and alpha-inhibin-92. Sequence analyses show that the NH2-terminal 31 amino acids of alpha-inhibin-52 are identical to the structure of the inhibin-like peptide previously reported [ILP-(1-31), now designated alpha-inhibin-31], and the COOH-terminal 52 amino acids of alpha-inhibin-92 are identical to the structure of alpha-inhibin-52. The amino acid sequence of alpha-inhibin-92 is: (sequence in text) Bioassay data in mouse pituitaries in vitro show that alpha-inhibin-52 is 3.4 times more active and alpha-inhibin-92 is greater than 40 times more active than alpha-inhibin-31 in suppressing follitropin-release. Radioimmunoassay data indicate that alpha-inhibin-52 and alpha-inhibin-92 have only 60% immunoreactivity.