Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors.
Susceptibility of lung epithelium to neutrophil elastase: protection by native inhibitors.
复制标题
肺上皮对中性粒细胞弹性酶的敏感性：天然抑制剂的保护。
DOI：
10.1080/09629359791488
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发表时间：
1997
影响因子：
4.6
通讯作者：
Tetley, T D
中科院分区：
文献类型：
--
作者：
Bingle, L;Richards, R J;Fox, B;Masek, L;Guz, A;Tetley, T D
肺气肿的发生被认为是由于蛋白酶（尤其是中性粒细胞弹性蛋白酶[NE]）和抗蛋白酶失衡所致，早期事件是呼吸道上皮松弛。我们在体外研究了在存在不同浓度和组合的天然抑制剂、α-1-蛋白酶抑制剂 (PI) 和分泌性白细胞蛋白酶抑制剂 (SLPI) 的情况下，NE 对呼吸道上皮细胞粘附的影响。 SLPI 在预防 NE 影响方面比 PI 有效 2 至 12 倍，特别是当酶与抑制剂的比例几乎相同时。即使 SLPI 的浓度仅为总量的 10%（如正常外周肺分泌物），它也比单独的 PI 提供更大的保护。这表明 SLPI 在控制中性粒细胞弹性蛋白酶诱导的炎症和组织损伤中发挥重要作用。
The development of emphysema is thought to be due to an imbalance of proteases (especially neutrophil elastase [NE]) and antiproteases with loosening of the respiratory epithelium as an early event. We investigated the effect of NE on respiratory epithelial cell adherence in vitro , in the presence of varying concentrations and combinations of native inhibitors, α-1-proteinase inhibitor (PI) and secretory leukoprotease inhibitor (SLPI). SLPI was two to 12 times more effective than PI at preventing the effects of NE, especially when enzyme:inhibitor ratios were almost equivalent. Even when the concentration of SLPI was only 10% of the total (as in normal peripheral lung secretions), it gave greater protection than PI alone. This suggests that SLPI plays an important role in controlling neutrophil elastaseinduced inflammation and tissue damage.