PirB-Cry2Aa hybrid protein exhibits enhanced insecticidal activity against Spodoptera exigua larvae
PirB-Cry2Aa hybrid protein exhibits enhanced insecticidal activity against Spodoptera exigua larvae
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PirB-Cry2Aa 杂合蛋白对甜菜夜蛾幼虫表现出增强的杀虫活性
DOI：
10.1016/j.jip.2014.05.004
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发表时间：
2014-07-01
影响因子：
3.4
通讯作者：
Hu, Shengbiao
中科院分区：
文献类型：
--
作者：
Hu, Xiaofeng;Liu, Zhengqiang;Hu, Shengbiao
发光杆菌PirB毒素的N-末端与苏云金芽孢杆菌Cry 2A毒素的N-末端结构域I有20.5%的同源性和41.5%的相似性。将Cry 2Aa蛋白的结构域I的编码序列替换为PirB蛋白的相应结构域的编码基因。pirB-cry 2Aa嵌合基因在B.苏云金杆菌无晶体菌株Cry（-）B导致形成具有不规则形状的晶体。生物测定结果表明，PirB-Cry 2Aa杂合蛋白对甜菜夜蛾和棉铃虫幼虫具有毒性。PirB蛋白可能具有致孔活性，PirB-Cry 2Aa杂合蛋白可作为生防剂。(C)2014 Elsevier Inc. All rights reserved.
Previous study revealed that the N-terminal region of PirB toxin from Photorhabdus luminescens showed 20.5% identity and 41.5% shnilarity to the domain I of Cry2A toxin of Bacillus thuringiensis. The encoding sequence of the domain I of Cry2Aa protein was replaced by the encoding gene of corresponding domain of PirB protein. Expression of pirB-cry2Aa chimeric gene in B. thuringiensis acrystalliferous strain Cry(-)B leads to the formation of crystals with irregular shape. Bioassay showed that PirB-Cry2Aa hybrid protein displayed toxicity against Spodoptera exigua and Helicoverpa armigera larvae. Our data implied that PirB protein might possess pore-forming activity and PirB-Cry2Aa hybrid protein could be used as biological control agent. (C) 2014 Elsevier Inc. All rights reserved.