Bacterial turgor pressure can be measured by atomic force microscopy
Bacterial turgor pressure can be measured by atomic force microscopy
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DOI：
10.1103/physreve.62.1034
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发表时间：
2000-07-01
影响因子：
2.4
通讯作者：
Boulbitch, A
中科院分区：
文献类型：
--
作者：
Arnoldi, M;Fritz, M;Boulbitch, A
本文报道了用原子力显微镜（AFM）研究细菌细胞壁的变形能力。一个理论表达式推导出的壁上的悬臂作为由AFM针尖产生的压痕的深度的函数所施加的力。提供的证据表明，这种反作用力是一种衡量的膨压的细菌。该方法被应用于趋磁细菌的物种Magnetocellum gryphiswaldense。在横向扫描时，在基底和细菌上产生力曲线。根据这样得到的力学性质，我们得到了细菌作为一个整体的弹簧常数。利用我们的理论结果，我们确定的膨压范围为85至150千帕。
We report a study of the deformability of a bacterial wall with an atomic force microscope (AFM). A theoretical expression is derived for the force exerted by the wall on the cantilever as a function of the depths of indentation generated by the AFM tip. Evidence is provided that this reaction force is a measure for the turgor pressure of the bacterium. The method was applied to magnetotactic bacteria of the species Magnetospirillum gryphiswaldense. Force curves were generated on the substrate and on the bacteria while scanning laterally. With the mechanical properties so gained we obtained the spring constant of the bacterium as a whole. Making use of our theoretical results we determined the turgor pressure to be in the range of 85 to 150 kPa.