The Diversity of Shell Matrix Proteins: Genome-wide Investigation of the Pearl Oyster, Pinctada fucata
The Diversity of Shell Matrix Proteins: Genome-wide Investigation of the Pearl Oyster, Pinctada fucata
复制标题
DOI：
10.2108/zsj.30.801
复制
发表时间：
2013-10-01
影响因子：
0.9
通讯作者：
Watabe, Shugo
中科院分区：
文献类型：
--
作者：
Miyamoto, Hiroshi;Endo, Hirotoshi;Watabe, Shugo
在软体动物中，壳基质蛋白与生物矿化有关，生物矿化是一种生物控制的过程，涉及碳酸钙晶体的成核和生长。壳基质蛋白的鉴定和表征对于更好地了解各种软体动物的适应性辐射是很重要的。我们搜索了珠母贝的基因组草稿序列，并注释了30种不同的壳基质蛋白。其中，Perarcin、室管膜蛋白相关蛋白和SPARC是双壳类和腹足类共有的基因；然而，大多数腹足类壳基质蛋白并不存在于岩藻基因组中。Glycinerich蛋白在珠母贝属中是保守的。关于这些注释基因的另一个重要发现是，许多壳基质蛋白由多个基因编码；例如，3个ACCBP样蛋白、3个CaLP、5个甲壳素合成酶样蛋白、2个N16蛋白(珍珠链)、10个N19蛋白、2个Nacrein、4个PIF、9个shematrins、2个prermarin-14蛋白和21个酪氨酸酶。贝壳基质蛋白的这种多样性可能与贝壳的形态多样性有关。本文所报道的注释基因可以在岩藻基因模型1.1版和基因组组装1.0版(http://marinegenomics.oist.jp/pinctada_fucata).中搜索到这些基因应该为研究生物矿化的遗传基础和评价壳基质蛋白作为软体动物进化工具包的作用提供有用的资源。
In molluscs, shell matrix proteins are associated with biomineralization, a biologically controlled process that involves nucleation and growth of calcium carbonate crystals. Identification and characterization of shell matrix proteins are important for better understanding of the adaptive radiation of a large variety of molluscs. We searched the draft genome sequence of the pearl oyster Pinctada fucata and annotated 30 different kinds of shell matrix proteins. Of these, we could identified Perlucin, ependymin-related protein and SPARC as common genes shared by bivalves and gastropods; however, most gastropod shell matrix proteins were not found in the P. fucata genome. Glycinerich proteins were conserved in the genus Pinctada. Another important finding with regard to these annotated genes was that numerous shell matrix proteins are encoded by more than one gene; e.g., three ACCBP-like proteins, three CaLPs, five chitin synthase-like proteins, two N16 proteins (pearlins), 10 N19 proteins, two nacreins, four Pifs, nine shematrins, two prismalin-14 proteins, and 21 tyrosinases. This diversity of shell matrix proteins may be implicated in the morphological diversity of mollusc shells. The annotated genes reported here can be searched in P. fucata gene models version 1.1 and genome assembly version 1.0 (http://marinegenomics.oist.jp/pinctada_fucata). These genes should provide a useful resource for studies of the genetic basis of biomineralization and evaluation of the role of shell matrix proteins as an evolutionary toolkit among the molluscs.