CAPILLARY ZONE ELECTROPHORESIS - EFFECT OF PHYSICAL PARAMETERS ON SEPARATION EFFICIENCY AND QUANTITATION
CAPILLARY ZONE ELECTROPHORESIS - EFFECT OF PHYSICAL PARAMETERS ON SEPARATION EFFICIENCY AND QUANTITATION
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DOI：
10.1002/jhrc.1240080810
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
JORGENSON, JW
中科院分区：
文献类型：
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作者：
LUKACS, KD;JORGENSON, JW
研究了硼硅玻璃毛细管/磷酸盐缓冲体系毛细管区带电泳分离效率与毛细管长度、直径和溶质浓度的关系。在恒定的施加功率（0.333 W）下，测量了硼硅酸盐、熔融二氧化硅和聚四氟乙烯毛细管在3-8的有效工作pH范围内的电渗透流动系数。通过简单、低色散的电迁移方法引入样品是一种可靠的溶质定量方法。
Separation efficiency in capillary zone electrophoresis was examined as a function of capillary length and diameter as well as solute concentration for a borosilicate glass capillary/phosphate buffer system. Electroosmotic flow coefficients in borosilicate, fused silica, and teflon capillaries were measured over the useful operating pH range of 3–8 at constant applied power (0.333 W). Sample introduction by a simple, low‐dispersion electromigration method was found to be a reliable procedure for solute quantitation.