E. coli enhance colonization resistance against Salmonella Typhimurium by competing for galactitol, a context-dependent limiting carbon source
E. coli enhance colonization resistance against Salmonella Typhimurium by competing for galactitol, a context-dependent limiting carbon source
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DOI：
10.1016/j.chom.2021.09.004
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发表时间：
2021-11-10
影响因子：
30.3
通讯作者：
Stecher, Barbel
中科院分区：
文献类型：
--
作者：
Eberl, Claudia;Weiss, Anna S.;Stecher, Barbel
固有微生物群落的组成决定了入侵的病原体是否会找到合适的定植环境并引起感染或被消灭。在这里，我们调查如何Escherosal E。大肠杆菌介导对鼠伤寒沙门氏菌（S. Tm）。使用合成的细菌群落，我们表明，E。coliMt1B1进行封闭S. TM定殖取决于微生物环境。在允许感染的情况下，E. coli利用了高度多样的碳源，不能阻断S.入侵。在小鼠中，稳定地定殖有12种遗传多样性的鼠肠道细菌（OMM 12），建立保护性环境，E.大肠杆菌耗尽的半乳糖醇，否则燃料S的底物。Tm殖民化。在这里，能够消耗C5和C6糖的毛螺菌科对CR有重要贡献。我们建议E.大肠杆菌通过消耗有限的碳源来提供CR，当处于善于从肠道中去除单糖的微生物群落中时。
The composition of intrinsic microbial communities determines if invading pathogens will find a suitable niche for colonization and cause infection or be eliminated. Here, we investigate how commensal E. coli mediate colonization resistance (CR) against Salmonella Typhimurium (S. Tm). Using synthetic bacterial communities, we show that the capacity of E. coli Mt1B1 to block S. Tm colonization depends on the microbial context. In an infection-permissive context, E. coli utilized a high diversity of carbon sources and was unable to block S. Tm invasion. In mice that were stably colonized by twelve phylogenetically diverse murine gut bacteria (OMM12), establishing a protective context, E. coli depleted galactitol, a substrate otherwise fueling S. Tm colonization. Here, Lachnospiraceae, capable of consuming C5 and C6 sugars, critically contributed to CR. We propose that E. coli provides CR by depleting a limited carbon source when in a microbial community adept at removing simple sugars from the intestine.