ANALYSIS OF BACTERIOPHAGE DEOXYRIBONUCLEIC-ACID SEQUENCES METHYLATED BY HOST-CONTROLLED AND R-FACTOR-CONTROLLED ENZYMES
ANALYSIS OF BACTERIOPHAGE DEOXYRIBONUCLEIC-ACID SEQUENCES METHYLATED BY HOST-CONTROLLED AND R-FACTOR-CONTROLLED ENZYMES
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DOI：
10.1128/jb.123.2.768-770.1975
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发表时间：
1975-01-01
影响因子：
3.2
通讯作者：
HATTMAN, S
中科院分区：
文献类型：
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作者：
MAY, MS;HATTMAN, S
λ和fd噬菌体在具有宿主K-12或N-3 R-因子脱氧核糖核酸-胞嘧啶甲基化酶活性的大肠杆菌菌株中繁殖。分析了含有3 H标记的5-甲基胞嘧啶（MeC）的嘧啶片段;在所有情况下，主要的甲基化序列是5'端。C-MeC-T ... 3'.
Phages lambda and fd were propagated in Escherichia coli strains that have either host K-12 or the N-3 R-factor deoxyribonucleic acid-cytosine methylase activity. Pyrimidine tracts containing 3H-labeled 5-methylcytosine (MeC) were analyzed; in all cases, the major methylated sequence was 5' ... C-MeC-T ... 3'.