Analysis of nephron composition and function in the adult zebrafish kidney.
Analysis of nephron composition and function in the adult zebrafish kidney.
复制标题
DOI：
10.3791/51644
复制
发表时间：
2014-08-09
期刊：
影响因子：
--
通讯作者：
Wingert RA
中科院分区：
文献类型：
--
作者：
McCampbell KK;Springer KN;Wingert RA
斑马鱼模型已成为研究肾脏发育、再生和疾病的相关系统。胚胎和成年斑马鱼的肾脏都是由称为肾元的功能单位组成的，这与其他脊椎动物包括哺乳动物一样高度保守。最近对斑马鱼的研究表明，成年肾单位受损后会发生两种不同的现象：第一，现有肾单位内存在强大的再生，取代被破坏的小管上皮细胞；第二，全新的肾元在肾祖细胞中产生，这一过程被称为新肾发生。相比之下，人类和其他哺乳动物似乎只有有限的肾细胞上皮再生能力。迄今为止，对这些肾脏再生现象的机制仍然知之甚少。由于成年斑马鱼的肾脏经历了肾细胞上皮再生和新肾形成，它们为研究这些事件提供了一个杰出的实验范例。此外，在斑马鱼模型中有广泛的遗传和药理学工具可用于描述调节肾脏再生的细胞和分子机制。这类研究的一个重要方面是评价肾元的结构和功能。本协议描述了一套标记技术，可用于测量肾脏组成和测试成年斑马鱼肾脏的肾元功能。因此，这些方法广泛适用于成年斑马鱼肾损伤模式的未来表型表征，包括但不限于肾毒性暴露机制或靶向细胞死亡的遗传方法，如硝基还原酶介导的细胞消融技术。此外，这些方法可用于研究成人肾脏形成中的遗传扰动，也可用于评估慢性疾病建模期间的肾脏状态。
The zebrafish model has emerged as a relevant system to study kidney development, regeneration and disease. Both the embryonic and adult zebrafish kidneys are composed of functional units known as nephrons, which are highly conserved with other vertebrates, including mammals. Research in zebrafish has recently demonstrated that two distinctive phenomena transpire after adult nephrons incur damage: first, there is robust regeneration within existing nephrons that replaces the destroyed tubule epithelial cells; second, entirely new nephrons are produced from renal progenitors in a process known as neonephrogenesis. In contrast, humans and other mammals seem to have only a limited ability for nephron epithelial regeneration. To date, the mechanisms responsible for these kidney regeneration phenomena remain poorly understood. Since adult zebrafish kidneys undergo both nephron epithelial regeneration and neonephrogenesis, they provide an outstanding experimental paradigm to study these events. Further, there is a wide range of genetic and pharmacological tools available in the zebrafish model that can be used to delineate the cellular and molecular mechanisms that regulate renal regeneration. One essential aspect of such research is the evaluation of nephron structure and function. This protocol describes a set of labeling techniques that can be used to gauge renal composition and test nephron functionality in the adult zebrafish kidney. Thus, these methods are widely applicable to the future phenotypic characterization of adult zebrafish kidney injury paradigms, which include but are not limited to, nephrotoxicant exposure regimes or genetic methods of targeted cell death such as the nitroreductase mediated cell ablation technique. Further, these methods could be used to study genetic perturbations in adult kidney formation and could also be applied to assess renal status during chronic disease modeling.
登录
查看更多内容
影响因子：
2.7
作者：
Li, Yue;Cheng, Christina N.;Verdun, Valerie A.;Wingert, Rebecca A.
通讯作者：
Wingert, Rebecca A.
DOI：
10.1097/01.asn.0000067645.49562.09
发表时间：
2003-06-01
影响因子：
13.6
作者：
Elger, M;Hentschel, H;Haller, H
通讯作者：
Haller, H
影响因子：
19.6
作者：
Bucaloiu, Ion D.;Kirchner, H. Lester;Perkins, Robert M.
通讯作者：
Perkins, Robert M.
DOI：
10.1097/01.asn.0000067652.51441.21
发表时间：
2003-06-01
影响因子：
13.6
作者：
Bonventre, JV
通讯作者：
Bonventre, JV
影响因子：
1.2
作者：
Cheng, Christina N.;Li, Yue;Wingert, Rebecca A.
通讯作者：
Wingert, Rebecca A.