Small animal, whole brain fMRI: Innocuous and nociceptive forepaw stimulation
Small animal, whole brain fMRI: Innocuous and nociceptive forepaw stimulation
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DOI：
10.1016/j.neuroimage.2006.12.014
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发表时间：
2007-04-01
期刊：
影响因子：
5.7
通讯作者：
Williams, Steve C. R.
中科院分区：
文献类型：
--
作者：
Lowe, Andrew S.;Beech, John S.;Williams, Steve C. R.
脊髓上疼痛处理涉及许多广泛的网络。使用小动物功能磁共振成像（fMRI）检查这些网络是很困难的。虽然之前的研究已经成功地描绘了与已知疼痛处理路径一致的区域，但它们仅限于以粗面内分辨率采集有限的空间范围，以实现高时间分辨率。已开发出一种各向同性全脑功能磁共振成像协议，用于检查大鼠前爪无害和伤害性电刺激的脊髓上后果。对大鼠前爪进行无害的电刺激描绘出与已知体感处理途径一致的大胆对比反应（对侧初级体感皮层（SI），与次级体感皮层一致的区域，腹侧后外侧丘脑核和同侧楔形核），为该技术提供了表面有效性。假定的有害刺激描绘了与经典的外侧和内侧疼痛系统以及次要相关区域一致的附加区域：厌恶和下降抑制系统。这些包括同侧下丘、前顶盖核、丘脑内侧核，以及前额叶、扣带回、腹侧眶和边缘下皮质、伏隔核的区域均表现出阴性 BOLD 变化。这些区域与先前报道的区域一致并延伸。本研究中采用的采集、后处理和分析方法构成了先前大鼠功能磁共振成像的显着扩展，使全脑覆盖的空间分辨率足以描绘与体感和伤害性网络一致的 BOLD 对比度的区域变化。 (c) 2006 Elsevier Inc. 保留所有权利。
Supra-spinal pain processing involves a number of extensive networks. An examination of these networks using small animal functional magnetic resonance imaging (fMRI) is difficult. While prior studies have successfully delineated regions consistent with known pain processing pathways, they have been restricted to acquisitions of limited spatial extent with coarse in-plane resolution to achieve a high temporal resolution. An isotropic, whole brain fMRI protocol has been developed for the examination of the supra-spinal consequences of innocuous and nociceptive electrical stimulation of the rat forepaw. Innocuous electrical stimulation of the rat forepaw delineated BOLD contrast responses consistent with known somatosensory processing pathways (contralateral primary somatosensory cortex (SI), a region consistent with secondary somatosensory cortex, the ventral posterolateral thalamic nucleus and ipsilateral cuneate nucleus), providing face validity for the technique. The putative noxious stimulus delineated additional regions consistent with the classical lateral and medial pain systems as well as secondarily associated areas: the aversion and descending inhibition systems. These included the ipsilateral inferior colliculus, anterior pretectal nucleus, mediodorsal thalamic nucleus, with regions in the pre-frontal, cingulated, ventral orbital and infra-limbic cortices, nucleus accumbens all exhibiting negative BOLD changes. Such regions are in agreement with, and extend, those previously reported. Acquisition, post-processing and analysis methodologies undertaken in this study constitute a marked extension of previous fMRI in the rat, enabling whole brain coverage at a spatial resolution sufficient to delineate regional changes in BOLD contrast consistent with somatosensory and nociceptive networks. (c) 2006 Elsevier Inc. All rights reserved.