AFM nanoindentation detection of the elastic modulus of tongue squamous carcinoma cells with different metastatic potentials.
AFM nanoindentation detection of the elastic modulus of tongue squamous carcinoma cells with different metastatic potentials.
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DOI：
10.1016/j.nano.2013.04.001
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
Zhuolong Zhou;Chaoxu Zheng;Su Li;Xiaofeng Zhou;Zhonghua Liu;Qianting He;Ningning Zhang;A. Ngan;B. Tang;Anxun Wang
中科院分区：
文献类型：
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作者：
Zhuolong Zhou;Chaoxu Zheng;Su Li;Xiaofeng Zhou;Zhonghua Liu;Qianting He;Ningning Zhang;A. Ngan;B. Tang;Anxun Wang
虽然在了解影响舌鳞状细胞癌（TSCC）转移的分子机制方面取得了重大进展，但对细胞弹性模量与TSCC转移之间的关系知之甚少。采用原子力显微镜（AFM）纳米压痕技术，通过速率跳跃法检测不同转移潜能的TSCC细胞和不同转移潜能的TSCC细胞系的弹性模量。与具有较低转移潜能的TSCC细胞相比，具有较高转移潜能的TSCC细胞显示弹性模量降低。弹性模量的降低还伴随着上皮-间质转化（EMT）、细胞骨架（F-actin和β-tubulin）的改变、细胞核变小和核质比（N/C）增大。本研究结果表明，细胞弹性模量与TSCC的转移密切相关。AFM纳米压痕通过速率跳跃法检测的弹性模量可以潜在地用于对TSCC的转移潜力进行分级。来自临床编辑这组研究人员报告了使用基于原子力显微镜的方法来确定舌鳞状细胞癌细胞的弹性模量，并证明了与具有较低转移潜能的细胞相比，具有较高转移潜能的这种细胞显示出降低的弹性模量。
Although significant advances have been made in understanding the molecular mechanisms that influence tongue squamous cell carcinoma (TSCC) metastasis, less is known about the association between the cellular elastic modulus and TSCC metastasis. Atomic force microscopy (AFM) nanoindentation via the rate-jump method was used to detect the elastic modulus of TSCC cells from patients and cell lines with different metastatic potentials. TSCC cells with higher metastatic potential showed decreases in the elastic modulus compared to TSCC cells with lower metastatic potential. Moreover, the decrease in elastic modulus was accompanied with epithelial–mesenchymal transition (EMT), cytoskeleton (F-actin and β-tubulin) changes, small nucleus size and large nucleus/cytoplasm (N/C) ratio. The present findings demonstrate a close relationship between the cellular elastic modulus and the metastasis of TSCC. The elastic modulus detected by AFM nanoindentation via the rate-jump method can potentially be used to grade the metastatic potential of TSCC.From the Clinical EditorThis team of investigators report the use of an atomic force microscopy-based method to determine the elastic modulus of tongue squamous cell carcinoma cells, and demonstrate that such cells with higher metastatic potential show decreased elastic modulus compared to cells with lower metastatic potential.