Antioxidant activities and lipid peroxidation status in human follicular fluid: age-dependent change
Antioxidant activities and lipid peroxidation status in human follicular fluid: age-dependent change
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DOI：
10.1017/s0967199421000241
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发表时间：
2021-12-01
期刊：
影响因子：
1.7
通讯作者：
Cadi, R.
中科院分区：
文献类型：
--
作者：
Debbarh, H.;Louanjli, N.;Cadi, R.
母亲年龄是影响体外受精（IVF）结果的重要因素。氧化应激（OS）是与年龄相关的细胞和分子损伤的主要原因之一。这项工作的目的是调查母亲年龄与卵泡内抗氧化剂和卵泡液 (FF) 中 OS 标记物之间的相关性，并确定高龄患者的 OS 状态。这项研究是一项前瞻性研究，纳入了 201 名年龄在 24 至 45 岁之间接受 IVF 的女性。 FF 样本是在卵母细胞取出时从成熟卵泡中获取的。 FF处理后，使用分光光度法评估脂质过氧化水平（MDA）和酶活性，例如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和谷胱甘肽（GSH）水平。结果表明，增加 MDA 水平的年龄截止点固定为 37 岁，使研究可以分为两个年龄组。 I组包括年龄小于37岁的患者，II组包括年龄大于或等于37岁的患者。统计分析显示，II组的MDA和GSH水平以及GR活性显着高于I组。II组的SOD和CAT活性显着低于I组。我们得出的结论是，从37岁开始，生殖衰老伴随着FF抗氧化模式的变化，从而损害了活性氧的清除效率。
Maternal age is a significant factor influencing in vitro fertilization (IVF) outcomes. Oxidative stress (OS) is one of the major causes of age-related cellular and molecular damage. The purpose of this work was to investigate the correlation between maternal age with intrafollicular antioxidants and OS markers in follicular fluid (FF), and also to determine the OS status in patients of advanced age. This study was a prospective study including 201 women undergoing IVF whose age was between 24 and 45 years old. FF samples were obtained from mature follicles at the time of oocyte retrieval. After treatment of FF, lipid peroxidation levels (MDA) and enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione (GSH) level were evaluated using spectrophotometry. The results indicated that the age cutoff point for increasing the MDA level was fixed at 37 years, allowing the study to be differentiated into two age groups. Group I included patients whose age was less than 37 years, and group II included patients whose age was greater than or equal 37 years. Statistical analysis revealed thatMDAand GSH levels and GR activity were significantly higher in group II compared with group I. The SOD and CAT activities were significantly less in group II compared with group I. We concluded that from 37 years old a reproductive ageing was accompanied by a change in the antioxidant pattern in FF that impaired reactive oxygen species scavenging efficiency.