Topographical and cytopathological lesion analysis of the white matter in Binswanger's disease brains
Topographical and cytopathological lesion analysis of the white matter in Binswanger's disease brains
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DOI：
10.1007/s00401-004-0850-2
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发表时间：
2004-06-01
影响因子：
12.7
通讯作者：
Budka, H
中科院分区：
文献类型：
--
作者：
Akiguchi, I;Tomimoto, H;Budka, H
本研究的目的是记录Binswanger病（BD）脑内白色物质（WM）的局部和细胞病理学病变。通过Kluver-Barrera染色，使用分级评分，对临床病理学证实的BD脑中与内侧丘脑和海马结构相关的各叶皮质下WM病变和纤维束病变进行评价。病变额皮质下WM的BD大脑，大脑从非神经系统患者，脑出血或大的皮质梗死也进行了化学检查，使用分子标记物轴突流损伤：淀粉样前体蛋白（APP）和脱髓鞘轴突病：致脑炎肽（EP）。我们的研究结果表明，WM病变在BD是显着更突出的额叶脑室周围和皮质下区域相比，其他皮质下WM病变，在顶叶，枕叶和颞叶的顺序。与其他束病变相比，在BD脑中，包括丘脑前脚在内的囊状核中的纤维束病变也显著更突出。此外，由EP抗血清和APP免疫反应性纤维标记的受损神经纤维的频率在BD脑中显著高于对照脑。WM损伤的分级分数与所有大脑中APP和EP免疫反应纤维的分级分数显著相关，包括对照组大脑。在我们的研究中，使用APP和EP的免疫组织化学清楚地显示了BD脑的额叶WM病变中的轴突损伤。
The purpose of the present study was to document the topographical and cytopathological lesions in the white matter (WM) of Binswanger's disease (BD) brains. Subcortical WM lesions in each lobe and fiber bundle lesions related to the medial thalamic and hippocampal structures in clinicopathologically proven BD brains were evaluated by Kluver-Barrera staining using a grading score. Lesions in the frontal subcortical WM of BD brains, brains from non-neurological patients, and brains with cerebral hemorrhage or large cortical infarcts were also examined immunohistochemically using molecular markers for axonal flow damage: amyloid precursor protein (APP); and for demyelinating axonopathy: encephalitogenic peptide (EP). Our results indicated that the WM lesions in BD were significantly more prominent in the frontal periventricular and subcortical regions as compared with other subcortical WM lesions, in the order of the parietal, occipital and temporal lobes. Fiber bundle lesions in the capsular genu, including the anterior thalamic peduncle, were also significantly more prominent in BD brains as compared with the other bundle lesions. Furthermore, the frequency of damaged nerve fibers labeled by the EP antiserum and APP immunoreactive fibers was significantly higher in BD brains as compared with the control brains. The grading scores for the WM damage correlated significantly with those for the APP and EP immunoreactive fibers in all brains, including the control brains. The axonal damage in the frontal WM lesions of the BD brains was clearly revealed in our study using immunohistochemistry for APP and EP.