Effects of hormone and growth factor on formation of fibroblast-aggregates for tissue-engineered skin
Effects of hormone and growth factor on formation of fibroblast-aggregates for tissue-engineered skin
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激素和生长因子对组织工程皮肤成纤维细胞聚集体形成的影响
DOI：
10.1016/s0928-4931(01)00337-x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Tateishi
中科院分区：
文献类型：
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作者：
K. Furukawa;T. Ushida;K. Kunii;Y. Sakai;J. Tanaka;T. Tateishi
为了加速组织工程皮肤产品植入后的伤口愈合，高密度的成纤维细胞接种将是重要的。因此，我们试图开发一种组织工程皮肤，通过形成细胞聚集体与生物可降解聚合物相结合，使成纤维细胞密度更高。我们的方法包括在未处理的培养皿中旋转摇动，减少成纤维细胞与材料的相互作用，增加细胞与细胞的相互作用。在培养条件下，正常新生人真皮成纤维细胞形成球状聚集体。在基础培养基中加入胰岛素、地塞米松、碱性成纤维细胞生长因子和抗坏血酸，并控制摇动时间，可加速成纤维细胞聚集体的形成。在基础培养基下形成的成纤维细胞聚集体中，当添加激素和生长因子时，与伤口愈合相关的基因表达水平高于单层。因此，具有聚集体和支架的组织工程皮肤产品将成为具有更高创面愈合潜力的人造皮肤。
To accelerate wound healing after implantation of tissue-engineered skin products, higher density inoculation of fibroblasts would be important. Therefore, we attempted to develop a tissue-engineered skin with a higher density of fibroblasts by formation of cell aggregates combined with a biodegradable polymer. Our method consisted of rotationally shaking with nontreated dishes, declining fibroblast-material interactions, and augmenting cell–cell interaction. Under the culture condition, normal neonatal human dermal fibroblasts formed spheroidal aggregates. The formation of the fibroblast-aggregates was accelerated by the addition of insulin, dexamethasone, basic fibroblast growth factor, and ascorbic acid to basal medium, and by the control of shaking time. In fibroblast-aggregates formed under a basal medium, the level of gene expression related to wound healing was higher than that of a monolayer when one added the hormones and growth factors. Therefore, tissue-engineered skin products with aggregates and scaffolds would become an artificial skin with potentiality of higher wound healing.