Analysis of peptides, proteins, protein digests, and whole human blood by capillary electrophoresis electrospray ionization mass spectrometry using an in-capillary electrode sheathless interface
Analysis of peptides, proteins, protein digests, and whole human blood by capillary electrophoresis electrospray ionization mass spectrometry using an in-capillary electrode sheathless interface
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DOI：
10.1016/s1044-0305(98)00081-6
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发表时间：
1998-10-01
影响因子：
3.2
通讯作者：
Moini, M
中科院分区：
文献类型：
--
作者：
Cao, P;Moini, M
将毛细管内无鞘电极接口应用于小分子肽、蛋白质和蛋白质的胰蛋白酶解产物的毛细管电泳/电喷雾质谱（CE/ESI-MS）分析。研究了不同实验参数对该接口性能的影响。在所用的实验条件下，毛细管内电极与CE出口的距离和毛细管内电极的长度对CE分离和ESI行为没有显著影响。然而，显着增强的灵敏度导致使用较窄的CE毛细管。使用四极杆质谱仪，氨基丙基硅烷涂层的毛细管，和宽扫描质荷比范围为500-1400，检测限约为4，1，和0.6 fmol细胞色素c和肌红蛋白分别达到75-，50-和30-μ m内径毛细管。在多离子监测模式下，实现了约一个数量级的检测下限。毛细管内电极无鞘界面对真实世界样品的应用通过人血液样品的CE/ESI-MS分析来证明，（C）1998年美国质谱学会。
An in-capillary electrode sheathless interface was applied to the capillary electrophoresis/electrospray ionization-mass spectrometry (CE/ESI-MS) analysis of mixtures of small peptides, proteins, and tryptic digests of proteins. The effects of different experimental parameters on the performance of this CE/ESI-MS interface were studied. The distance of the in-capillary electrode from the CE outlet and the length of the electrode inside the capillary had no significant effects on the CE separation and ESI behavior under the experimental conditions used. However, significant enhancement of the sensitivity resulted from the use of narrower CE capillaries. Using a quadrupole mass spectrometer, an aminopropylsilane-coated capillary, and a wide scan mass-to-charge ratio range of 500-1400, detection limits of approximately 4, 1, and 0.6 fmol for cytochrome c and myoglobin were achieved for 75-, 50-, and 30-mu m inner diameter capillaries, respectively. Approximately one order of magnitude lower detection limits were achieved under the multiple-ion monitoring mode. The application of the in-capillary electrode sheathless interface to real-world samples was demonstrated by CE/ESI-MS analysis of a human blood sample, (C) 1998 American Society for Mass Spectrometry.