SEROLOGICAL COMPARISON OF 10 GLYCOLYTIC MYCOPLASMA SPECIES
SEROLOGICAL COMPARISON OF 10 GLYCOLYTIC MYCOPLASMA SPECIES
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DOI：
10.1128/jb.98.3.1044-1055.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
KENNY, GE
中科院分区：
文献类型：
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作者：
KENNY, GE
17株支原体代表11个命名的物种进行了比较血清学的三个参数：生长抑制琼脂，双向免疫扩散，补体结合。在生长抑制研究中，发现一株标记为嗜组织支原体的菌株与M有关，可能最好归类为M。肺，免疫双扩散研究证实的关系。对其余10个物种的比较表明，两对物种可以通过补体结合和双向免疫扩散而不是通过生长抑制来显示密切相关;它们是：M.粒壳M.莱德拉威兰和M.猫M犬。M. pneumoniae和M.鸡败血症是本研究中血清学上最独特的微生物，相互之间或与其他种属之间几乎没有交叉反应。总体而言，比较血清学获得的分类分组似乎与支原体脱氧核糖核酸的鸟嘌呤加胞嘧啶含量作为分类标准时获得的分组相关。鸟嘌呤加胞嘧啶含量为23%至28%的一组生物体（M。canis，M. fermentans，M. hyorhinis，M. neurolyticum和M.肺结核）似乎通常与血清学相关。因此，在支原体目脱氧核糖核酸的碱基组成中观察到的显著异质性也反映了相应的血清学异质性，并显然被其掩盖。
Seventeen strains of mycoplasmata representing 11 named species were compared serologically by three parameters: growth inhibition on agar, double immunodiffusion, and complement fixation. In growth-inhibition studies, a strain labeledMycoplasma histotropicumwas found related to and perhaps best classified asM. pulmonis, a relationship confirmed by double immuno-diffusion studies. A comparison of the remaining 10 species demonstrated that two pairs of species could be shown to be closely related by complement fixation and double immunodiffusion but not by growth inhibition; these were:M. granularum-M. laidlawiiandM. felis-M. canis. M. pneumoniaeandM. gallisepticumwere the most serologically unique organisms in this study, showing very few cross-reactions with each other or other species. Overall, taxonomic groupings obtained by comparative serology appeared to correlate with the groupings obtained when the guanine plus cytosine contents of the deoxyribonucleic acid of mycoplasmata were employed as classification criteria. The group of organisms having a guanine plus cytosine content of 23 to 28% (M. canis, M. fermentans, M. hyorhinis, M. neurolyticum, andM. pulmonis) appeared to be generally serologically related. Thus the remarkable heterogeneity observed in the base composition of the deoxyribonucleic acid of orderMycoplasmatalesis also reflected and apparently paralleled by a corresponding serological heterogeneity.