THERMOTOLERANCE AND SYNTHESIS OF HEAT-SHOCK PROTEINS - THESE RESPONSES ARE PRESENT IN HYDRA-ATTENUATA BUT ABSENT IN HYDRA-OLIGACTIS
THERMOTOLERANCE AND SYNTHESIS OF HEAT-SHOCK PROTEINS - THESE RESPONSES ARE PRESENT IN HYDRA-ATTENUATA BUT ABSENT IN HYDRA-OLIGACTIS
复制标题
DOI：
10.1073/pnas.85.21.7927
复制
发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
STEELE, RE
中科院分区：
文献类型：
--
作者：
BOSCH, TCG;KRYLOW, SM;STEELE, RE
生物体通过合成少量高度保守的热休克蛋白来应对环境胁迫。在细菌、植物、无脊椎动物和脊椎动物等多种生物体中，这些蛋白质的合成与获得耐热性直接相关。在研究淡水腔肠动物水螅的过程中，我们观察到水螅对热胁迫非常敏感。相反，相关物种Hydra attenuata在短期暴露于高温下生存。此外，在升高的但非致死的温度下孵育后，H. oligactis没有变得耐热。H.然而，attenuata在这样的预孵育后获得了耐热性。In H. attenuata的主要热休克蛋白的大小为60 kDa。H. oligactis没有合成可检测水平的这种蛋白质或任何新种类的蛋白质来响应压力。其他几种水螅也被发现有类似H的行为。oligactis反应压力。因此，这些研究结果提供了直接支持的假设，热休克蛋白是所需的胁迫耐受性和主要的热休克蛋白在水螅没有任何影响正常的生长或生理。研究结果还表明，热休克反应的存在可能与生物体生活的自然环境有关。
Organisms respond to environmental stress by synthesizing a small number of highly conserved heat shock proteins. In organisms as diverse as bacteria, plants, invertebrates, and vertebrates, synthesis of these proteins is directly correlated with the acquisition of thermotolerance. While studying the freshwater coelenterate hydra, we observed that Hydra oligactis was extremely sensitive to thermal stress. In contrast, the related species Hydra attenuata survives short-term exposure to high temperatures. Furthermore, after incubation at an elevated but nonlethal temperature, H. oligactis did not become thermotolerant. H. attenuata, however, acquired thermotolerance after such a preincubation. In H. attenuata the major heat shock protein was found to be 60 kDa in size. H. oligactis did not synthesize detectable levels of this protein or any new species of proteins in response to stress. Several other species of hydra were found to behave like H. oligactis in response to stress. Thus, these findings provide direct support for the hypothesis that heat shock proteins are required for stress tolerance and that the major heat shock protein in hydra does not have any effects on normal growth or physiology. The findings also indicate that the presence of a heatshock response might be related to the natural environment in which an organism lives.