Topical application of aloperine improves 2,4-dinitrofluorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice
Topical application of aloperine improves 2,4-dinitrofluorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice
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DOI：
10.1016/j.ejphar.2011.02.013
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发表时间：
2011-05-11
影响因子：
5
通讯作者：
Guo, Jian-You
中科院分区：
文献类型：
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作者：
Yuan, Xiao-Ying;Ma, Hui-Min;Guo, Jian-You
苦参碱对2，4-二硝基氟苯(DNFB)诱导的BALB/c小鼠变态反应性接触性皮炎有抑制作用。在本研究中，我们进一步研究了苦豆碱对DNFB诱导的NC/NGA小鼠特应性皮炎样皮肤病变的影响。外用DNFB后，NC/NGA小鼠出现特应性皮炎样皮肤病变。苦参碱治疗显著抑制DNFB处理的NC/NGA小鼠的皮炎指数和耳朵厚度，并呈剂量依赖关系。苦杏仁碱治疗还能抑制嗜酸性粒细胞、肥大细胞向耳内的渗透和血浆免疫球蛋白E水平。最后，经DNFB攻击后，耳活检组织匀浆中细胞因子(IL-1β、IL-4、IL-6、IL-10、IL-13、肿瘤坏死因子(TNF)-α和干扰素(干扰素)-γ)的产生显著增加。局部应用苦参碱可增加免疫抑制细胞因子IL-10的水平，同时以剂量依赖的方式减少其他细胞因子的产生。综上所述，这些数据表明苦豆碱可能是治疗特应性皮炎的有效药物之一。(C)2011爱思唯尔B.V.保留所有权利。
Aloperine has been shown to inhibit 2,4-dinitrofluorobenzene (DNFB) induced allergic contact dermatitis in BALB/c mice. In the present study, we further investigated the effect of aloperine on DNFB-induced atopic dermatitis-like skin lesions in NC/Nga mice. NC/Nga mice elicited atopic dermatitis-like skin lesions after the topical application of DNFB. Aloperine treatment significantly inhibited dermatitis index and ear thickness in DNFB-treated NC/Nga mice in a dose-dependent manner. Eosinophils, mast cells infiltration into the ears and plasma level of immunoglobulin (Ig) E were also suppressed by aloperine treatment. Finally, cytokine (interleukin (IL)-1 beta, IL-4, IL-6, IL-10, IL-13, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma) productions in ear biopsies homogenates were significantly elevated after DNFB challenge. Topical application of aloperine increased the immunosuppressive cytokine IL-10 level, while it reduced other cytokines production in a dose-dependent manner. Taken together, these data suggest that aloperine may be one of the effective therapeutic agents for the treatment of atopic dermatitis. (C) 2011 Elsevier B.V. All rights reserved.