Identification and localization of the CupB protein involved in constitutive CO2 uptake in the cyanobacterium, Synechocystis sp strain PCC 6803
Identification and localization of the CupB protein involved in constitutive CO2 uptake in the cyanobacterium, Synechocystis sp strain PCC 6803
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蓝藻集胞藻菌株 PCC 6803 中参与 CO2 吸收的 CupB 蛋白的鉴定和定位
DOI：
10.1093/pcp/pcn074
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发表时间：
2008-06-01
影响因子：
4.9
通讯作者：
Mi, Hualing
中科院分区：
文献类型：
--
作者：
Xu, Min;Ogawa, Teruo;Mi, Hualing
抗cMyc抗体与集胞藻PCC 6803类囊体膜上His 6-cMyc（HM）标记的CupB蛋白有较强的交叉反应，但与胞质膜组分的交叉反应较弱。在用HM标记CupB的Delta ndhD 4和Delta ndhF 4突变体的膜中未检测到蛋白质。我们的结论是，含有NdhD 4和NdhF 4的CupB复合物很大程度上（如果不是唯一的话）局限于类囊体膜。在含有>450 kDa的蛋白质复合物的级分中检测到CupB和NdhH两者，所述级分在镍柱和用正十二烷基-β-麦芽糖苷溶解的膜的凝胶过滤层析之后获得。
Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane fraction. The protein was not detected in the membranes of the Delta ndhD4 and Delta ndhF4 mutants in which CupB was tagged with HM. We concluded that a CupB complex containing NdhD4 and NdhF4 is largely, if not exclusively, confined to the thylakoid membrane. Both CupB and NdhH were detected in a fraction containing protein complexes of >450 kDa, obtained after nickel column and gel filtration chromatography of the membranes solubilized with n-dodecyl-beta-maltoside.