Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function.
Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function.
复制标题
纯化的酵母菌的聚合：Septin功能可能不需要有组织的丝阵列。
DOI：
10.1083/jcb.143.3.737
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发表时间：
1998-11-02
影响因子：
7.8
通讯作者：
Field, C
中科院分区：
文献类型：
--
作者：
Frazier, J A;Wong, M L;Longtine, M S;Pringle, J R;Mann, M;Mitchison, T J;Field, C
脓毒蛋白是许多真核细胞类型中胞质分裂所需的蛋白质家族。在芽殖酵母中，这些蛋白质被认为是存在于母芽颈部的细丝系统（称为颈细丝）的结构成分。在这项研究中，我们报告了含有酵母脓毒蛋白 Cdc3p、Cdc10p、Cdc11p 和 Cdc12p 的蛋白质复合物的分离，该蛋白质复合物能够在体外形成长丝。为了研究这些丝与颈丝之间的关系，我们从删除了 CDC10 或 CDC11 的细胞中纯化了脓毒蛋白复合物。这些复合物不能进行野生型制剂所表现出的聚合作用，并且通过电子显微镜对颈部区域的分析表明，cdc10Δ和cdc11Δ细胞不包含可检测到的颈部丝。这些结果强化了脓蛋白是颈丝的主要结构成分的假设。令人惊讶的是，我们发现 cdc10Δ 细胞中仍然发生诸如胞质分裂和 Bud4p 颈部定位等 Septin 依赖性过程。这表明脓毒症可能能够在没有正常聚合和形成更高阶丝结构的情况下发挥作用。
The septins are a family of proteins required for cytokinesis in a number of eukaryotic cell types. In budding yeast, these proteins are thought to be the structural components of a filament system present at the mother–bud neck, called the neck filaments. In this study, we report the isolation of a protein complex containing the yeast septins Cdc3p, Cdc10p, Cdc11p, and Cdc12p that is capable of forming long filaments in vitro. To investigate the relationship between these filaments and the neck filaments, we purified septin complexes from cells deleted for CDC10 or CDC11. These complexes were not capable of the polymerization exhibited by wild-type preparations, and analysis of the neck region by electron microscopy revealed that the cdc10Δ and cdc11Δ cells did not contain detectable neck filaments. These results strengthen the hypothesis that the septins are the major structural components of the neck filaments. Surprisingly, we found that septin dependent processes like cytokinesis and the localization of Bud4p to the neck still occurred in cdc10Δ cells. This suggests that the septins may be able to function in the absence of normal polymerization and the formation of a higher order filament structure.