Purification of messenger ribonucleoprotein particles from rabbit reticulocytes by zonal centrifugation in metrizamide.
Purification of messenger ribonucleoprotein particles from rabbit reticulocytes by zonal centrifugation in metrizamide.
复制标题
通过在甲曲酰胺中进行区带离心纯化兔网织红细胞中的信使核糖核蛋白颗粒。
DOI：
10.1111/j.1432-1033.1981.tb06195.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
J. Traugh
中科院分区：
文献类型：
--
作者：
D. Rittschof;J. Traugh
本文报道了一种从兔网织红细胞多聚核糖体中大规模纯化信使核糖核蛋白（mRNP）颗粒的方法。通过尿素处理使mRNP颗粒从多聚核糖体解离，并在高离子强度条件下通过差速离心分离。在甲泛葡胺浮力密度梯度中的区带离心是最后的纯化步骤。观察到一类主要的mRNA颗粒。通过聚丙烯酰胺凝胶电泳和在无细胞蛋白质合成系统中珠蛋白的产生将RNA定义为mRNA。与颗粒相关的23种不同蛋白质是一组离散的蛋白质，分子量从175，000到23，500不等。通过计算机模拟从染色蛋白质的凝胶扫描确定颗粒中每种肽的相对量。通过双向聚丙烯酰胺凝胶电泳分析，没有多肽与40-S和60-S核糖体亚基的蛋白质共迁移。
A large-scale purification procedure for messenger ribonucleoprotein (mRNP) particles from rabbit reticulocyte polysomes is described. The mRNP particles were dissociated from polysomes by treatment with urea and separated by differential centrifugation under conditions of high ionic strength. Zonal centrifugation in a metrizamide buoyant density gradient was the final purification step. One major class of mRNA particle was observed. The RNA was defined as mRNA by polyacrylamide gel electrophoresis and by globin production in a cell-free protein-synthesizing system. The twenty-three different proteins associated with the particle were a discrete set of proteins, which ranged in molecular weight from 175,000 to 23,500. The relative amount of each peptide in the particle was determined from a gel scan of the stained protein by computer simulation. None of the polypeptides comigrated with proteins from the 40-S and 60-S ribosomal subunits when analyzed by two-dimensional polyacrylamide gel electrophoresis.
转移核糖核酸的高分辨率磷核磁共振波谱。
影响因子：
4.3
作者：
Gorenstein,DG;Goldfield,EM
通讯作者：
Goldfield,EM