Immunohistochemical marker panels for distinguishing between epithelioid mesothelioma and lung adenocarcinoma
Immunohistochemical marker panels for distinguishing between epithelioid mesothelioma and lung adenocarcinoma
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DOI：
10.1111/j.1440-1827.2007.02080.x
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发表时间：
2007-04-01
影响因子：
2.2
通讯作者：
Inai, Kouki
中科院分区：
文献类型：
--
作者：
Kushitani, Kei;Takeshima, Yukio;Inai, Kouki
上皮样间皮瘤和肺腺癌之间的区别仍然是一个重要的诊断外科病理学家的挑战。本研究的目的是选择一个有限的和适当的面板的抗体，可以区分上皮样间皮瘤和肺腺癌。对90例上皮样间皮瘤和51例肺腺癌标本进行了钙视黄蛋白、WT 1、AE 1/AE 3、CAM 5.2、细胞角蛋白（CK）5/6、波形蛋白、上皮膜抗原（EMA）、血栓调节蛋白、CEA、CA 19 -9和CA 125检测。96%的上皮样间皮瘤的钙视蛋白阳性; 99%的WT 1阳性; 100%的AE 1/AE阳性; 97%的CAM 5.2阳性; 70%的CK 5/6阳性; 91%的波形蛋白阳性; 96%的EMA阳性; 71%的血栓调节蛋白阳性; 77%的间皮素阳性; 7%的CEA阳性; 17%的CA 19 -9阳性; 85%的CA 125阳性。相比之下，33%的肺腺癌对钙视黄蛋白呈阳性; 16%的肺腺癌对WT 1呈阳性; 100%的肺腺癌对AE 1/AE 3、CAM 5.2和EMA呈阳性; 41%的肺腺癌对CK 5/6呈阳性; 47%的肺腺癌对波形蛋白呈阳性; 20%的肺腺癌对血栓调节蛋白呈阳性; 69%的肺腺癌对间皮素呈阳性; 98%的肺腺癌对CEA呈阳性; 73%的肺腺癌对CA 19 -9呈阳性; 80%的肺腺癌对CA 125呈阳性。对于鉴别上皮样间皮瘤和肺腺癌，CEA、calretinin和WT 1或血栓调节蛋白的组合被认为是最好的免疫组化标记物。
The distinction between epithelioid mesothelioma and lung adenocarcinoma remains an important diagnostic challenge for surgical pathologists. The aim of the present study was to select a limited and appropriate panel of antibodies that can differentiate between epithelioid mesothelioma and lung adenocarcinoma. Specimens of 90 epithelioid mesotheliomas and 51 lung adenocarcinomas obtained from Japanese cases were examined using calretinin, WT1, AE1/AE3, CAM5.2, cytokeratin (CK) 5/6, vimentin, epithelial membrane antigen (EMA), thrombomodulin, CEA, CA19-9, and CA125. Ninety-six percent of epithelioid mesotheliomas were positive for calretinin; 99% for WT1; 100% for AE1/AE; 97% for CAM5.2; 70% for CK 5/6; 91% for vimentin; 96% for EMA; 71% for thrombomodulin; 77% for mesothelin; 7% for CEA; 17% for CA19-9; and 85% for CA125. In contrast, 33% of lung adenocarcinomas were positive for calretinin; 16% for WT1; 100% for AE1/AE3, CAM5.2, and EMA; 41% for CK 5/6; 47% for vimentin; 20% for thrombomodulin; 69% for mesothelin; 98% for CEA; 73% for CA19-9; and 80% for CA125. For distinguishing between epithelioid mesothelioma and lung adenocarcinoma, the combination of CEA, calretinin and each WT1 or thrombomodulin was suggested to be the best panel of immunohistochemical markers.