Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages
Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages
复制标题
通过外部扩容装置增加腺上皮细胞簇，通过招募巨噬细胞促进脂肪组织再生
DOI：
10.1042/bsr20181776
复制
发表时间：
2019-02-28
影响因子：
4
通讯作者：
Yuan, Yi
中科院分区：
文献类型：
--
作者：
Chen, Xihang;He, Yunfan;Yuan, Yi
背景：临床上需要使用工程化脂肪组织代替外科重建。我们以前发现，外部体积扩张（EVE）设备增加了特殊的细胞簇在血管化结缔间质在脂肪再生。然而，这些细胞簇的起源及其在脂肪组织再生中的作用仍然未知。目的：在本研究中，我们评估EVE在大鼠模型中构建扩张预制脂肪组织（EPAT）的作用。方法：将大鼠随机分为EVE吸引组和对照组，每组24只。特殊的细胞簇的结构和起源进行了确定苏木精和伊红染色，免疫组化，免疫组化和蛋白质印迹分析，它们在脂肪组织再生的作用进行了研究。结果：特殊细胞团在第1周开始增加，第4周达到高峰，第8 ~ 12周逐渐减少。根据其腺体样结构和特异性标志物的表达，将簇鉴定为腺上皮细胞。在抽吸的早期，细胞团通过分泌单核细胞趋化蛋白-1（MCP-1）诱导巨噬细胞抗原-2（Mac-2）阳性巨噬细胞的显著浸润。随后，这些浸润的巨噬细胞表达大量的血管内皮生长因子（VEGF），以促进血管生成。结论：EVE产生腺上皮细胞簇，其募集巨噬细胞以促进血管生成和随后的脂肪组织再生。这些发现阐明了EVE装置对脂肪组织再生的影响的潜在机制。
Background: There is a clinical need for the use of engineered adipose tissue in place of surgical reconstruction. We previously found that the external volume expansion (EVE) device increased special cell clusters in well-vascularized connective stroma during adipose regeneration. However, the origin of these cell clusters and their role in adipose tissue regeneration remain unknown. Aim: In the present study, we evaluated EVE in the construction of expanded prefabricated adipose tissue (EPAT) in a rat model. Methods: Rats were randomized into an EVE suction group and a control group, with 24 rats in each group. The structure and origin of the special cell clusters were determined by hematoxylin and eosin staining, and immunohistochemistry; their role in adipose tissue regeneration was investigated by immunohistochemistry and Western blot analyses. Results: Special cell clusters began to increase at week 1 with a peak at week 4, and then receded from weeks 8 to 12. Clusters were identified as glandular epithelial cells as determined by their gland-like structure and expression of specific markers. The cell clusters induced significant infiltration of macrophage antigen-2 (Mac-2) positive macrophages by secreting monocyte chemoattractant protein-1 (MCP-1) at the early stage of suction. Subsequently, these infiltrated macrophages expressed massive vascular endothelial growth factor (VEGF) to promoted angiogenesis. Conclusion: EVE generated glandular epithelial cell clusters, which recruited macrophages to promote angiogenesis and subsequent adipose tissue regeneration. These findings shed light on the mechanisms underlying the effects of EVE devices on adipose tissue regeneration.