Cardiac microdialysis to estimate interstitial adenosine and coronary blood flow.
Cardiac microdialysis to estimate interstitial adenosine and coronary blood flow.
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心脏微透析估计间质腺苷和冠状动脉血流量。
DOI：
10.1152/ajpheart.1990.258.6.h1642
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
MentzerJr,RM
中科院分区：
文献类型：
--
作者：
VanWylen,DG;Willis,J;Sodhi,J;Weiss,RJ;Lasley,RD;MentzerJr,RM
本研究有两个目的：1）研究心脏微透析用于同时估计局部心脏间质液（ISF）腺苷（ADO）浓度和冠状动脉血流量（CBF）的可行性和有用性; 2）确定心脏刺激或局部心肌缺血期间ADO和CBF的ISF水平的变化。将心脏微透析探针植入氯醛糖-氨基甲酸乙酯麻醉的狗的左心室心肌中，并用Krebs-Henseleit缓冲液灌注。流出透析液中ADO的浓度被用作心肌内ISF ADO浓度的指标，而局部CBF通过透析纤维内的铂丝通过H2清除来测量。透析液ADO在插入微透析探针后立即升高，在前20 min迅速下降，60 min稳定，并保持恒定2 h。根据微透析探头灌注速率与透析液ADO浓度之间的体外和体内关系，估计左心室心肌内ISF ADO浓度为0.9-1.3 μ M。多巴酚丁胺（10 μ g.kg-1.min-1）输注导致CBF增加36%，透析液ADO增加2.5倍（n = 9; P <0.05）。左前降支（LAD）闭塞诱导的局部心肌缺血导致LAD灌注心肌中透析液ADO增加13倍（n = 9; P <0.05）。这些结果与ADO假说一致，表明心脏微透析为区域心肌内ISF的采样提供了可靠的技术。
The purpose of this study was twofold: 1) to investigate the feasibility and usefulness of cardiac microdialysis for the simultaneous estimation of regional cardiac interstitial fluid (ISF) adenosine (ADO) concentration and coronary blood flow (CBF); and 2) to determine the changes in the ISF levels of ADO and CBF during cardiac stimulation or regional myocardial ischemia. Cardiac microdialysis probes were implanted in the left ventricular myocardium of chloralose-urethan-anesthetized dogs and perfused with Krebs-Henseleit buffer. The concentration of ADO in the effluent dialysate was used as an index of intramyocardial ISF ADO concentration while local CBF was measured by H2 clearance via a platinum wire within the dialysis fiber. Dialysate ADO was elevated immediately after insertion of the microdialysis probe, declined rapidly in the first 20 min, stabilized by 60 min, and remained constant for 2 h. Based on the relationship in vitro and in vivo between microdialysis probe perfusion rate and dialysate ADO concentration, ISF ADO concentration within the left ventricular myocardium was estimated to be 0.9-1.3 microM. Dobutamine (10 micrograms.kg-1.min-1) infusion resulted in a 36% increase in CBF and a 2.5-fold increase in dialysate ADO (n = 9; P less than 0.05). Regional myocardial ischemia, induced by occlusion of the left anterior descending artery (LAD), caused a 13-fold increase in dialysate ADO in the LAD perfused myocardium (n = 9; P less than 0.05). These results are consistent with the ADO hypothesis and suggest that cardiac microdialysis provides a reliable technique for the sampling of regional intramyocardial ISF.