Nutritional approach to restore impaired intestinal barrier function and growth after neonatal stress in rats
Nutritional approach to restore impaired intestinal barrier function and growth after neonatal stress in rats
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DOI：
10.1097/01.mpg.0000226376.95623.9f
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发表时间：
2006-07-01
影响因子：
2.9
通讯作者：
Corthesy-Theulaz, Irene
中科院分区：
文献类型：
--
作者：
Garcia-Rodenas, Clara L.;Bergonzelli, Gabriela E.;Corthesy-Theulaz, Irene
目的：新生儿期心理应激会导致日后肠屏障功能障碍和生长发育改变。方法：84只雄性Wistar幼鼠从出生后第2天至第14天被分配到2种饲养条件之一：S，与母亲分开3h/d，或H，15min/d处理对照组。从PND15到PND35，大鼠接受对照饲料或含有花生四烯酸和二十二碳六烯酸、低聚半乳糖和果寡糖以及副干酪乳杆菌NCC2461的类似饲料。结果：母亲的分离对PND15的肠道屏障参数的测量影响很小，而在PND35的影响很大。在这个年龄段，与H动物相比，S的肠道对大分子物质的通透性更高，小肠组织中的粘蛋白含量更低，微生物区系组成发生了变化。饲喂适应的饮食可使肠道通透性恢复正常，但不能恢复肠道粘蛋白含量或微生物区系。此外，适应饲料还能促进S动物断奶后的生长速度恢复，并导致小肠绒毛长度的增加。结论：添加特定的长链多不饱和脂肪酸、益生素和益生菌的适应饲料可以逆转新生儿应激对肠屏障功能和生长的负面影响。
Objectives: Psychological stress during the neonatal period results in intestinal barrier dysfunction and growth alterations later in life. We aimed to restore impaired barrier function and growth rate by a nutritional intervention.Methods: Male rat pups (n = 84) were assigned to 1 of 2 rearing conditions from postnatal day (PND) 2 to PND14: S, separated 3 h/d from their mothers, or H, 15 min/d handled controls. From PND15 to PND35, rats received a control diet or a similar diet adapted to contain arachidonic and docosahexaenoic acids, galacto- and fructo-oligosaccharides and Lactobacillus para-casei NCC2461.Results: Maternal separation had only a minor impact on the measured gut barrier parameters at PND15, whereas it severely affected them at PND35. At this age, intestinal permeability to macromolecules was higher, mucin content in small intestinal tissues was lower and microbiota composition was altered in S compared with H animals. Feeding the adapted diet normalized the intestinal permeability, although it did not restore intestinal mucin content or microbiota. In addition, the adapted diet improved the growth rate recovery of the S animals after weaning and resulted in increased villus length in small intestine.Conclusion: Our results suggest that an adapted diet containing specific long-chain polyunsaturated fatty acids, prebiotics and probiotics can revert the negative imprinting of neonatal stress on both intestinal barrier function and growth.