SMAUG is a major regulator of maternal mRNA destabilization in Drosophila and its translation is activated by the PAN GU kinase
SMAUG is a major regulator of maternal mRNA destabilization in Drosophila and its translation is activated by the PAN GU kinase
复制标题
DOI：
10.1016/j.devcel.2006.10.005
复制
发表时间：
2007-01-01
影响因子：
11.8
通讯作者：
Lipshitz, Howard D.
中科院分区：
文献类型：
--
作者：
Tadros, Wael;Goldman, Aaron L.;Lipshitz, Howard D.
在动物中，卵子激活触发了一系列转录后事件，这些事件作用于母体合成的RNA。我们发现，在果蝇中，PAN GU（PNG）激酶位于该级联的顶部附近，触发SMAUG（SMG）的翻译，SMAUG是一种从酵母到人类保守的多功能转录后调节因子。尽管PNG是smg mRNA的胞质聚腺苷酸化所必需的，但它通过独立于其对poly（A）尾的影响的机制调节翻译。突变体的分析表明，PNG解除了由PUM和一个或多个其他因子引起的翻译抑制，这些因子通过smg mRNA的3'非翻译区（UTR）平行作用。基于微阵列的基因表达谱分析表明，SMG是母体转录不稳定的主要调节因子。SMG依赖的mRNA的基因本体论注释丰富的细胞周期中的功能，这表明未能消除这些转录和细胞周期缺陷的SMG突变体之间的可能的因果关系。
In animals, egg activation triggers a cascade of posttranscriptional events that act on maternally synthesized RNAs. We show that, in Drosophila, the PAN GU (PNG) kinase sits near the top of this cascade, triggering translation of SMAUG (SMG), a multifunctional posttranscriptional regulator conserved from yeast to humans. Although PNG is required for cytoplasmic polyadenylation of smg mRNA, it regulates translation via mechanisms that are independent of its effects on the poly(A) tail. Analyses of mutants suggest that PNG relieves translational repression by PUMILIO (PUM) and one or more additional factors, which act in parallel through the smg mRNA's 3' untranslated region (UTR). Microarray-based gene expression profiling shows that SMG is a major regulator of maternal transcript destabilization. SMG-dependent mRNAs are enriched for gene ontology annotations for function in the cell cycle, suggesting a possible causal relationship between failure to eliminate these transcripts and the cell cycle defects in smg mutants.