DESORPTION OF PORCINE PARVOVIRUS FROM ALUMINUM HYDROXIDE ADJUVANT WITH SUBSEQUENT VIRAL IMMUNOASSAY OF HEMAGGLUTINATION ASSAY
DESORPTION OF PORCINE PARVOVIRUS FROM ALUMINUM HYDROXIDE ADJUVANT WITH SUBSEQUENT VIRAL IMMUNOASSAY OF HEMAGGLUTINATION ASSAY
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DOI：
10.1007/bf00361329
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发表时间：
1987-01-01
影响因子：
2.2
通讯作者：
KATZ, J
中科院分区：
文献类型：
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作者：
KATZ, J
用不同浓度的氢氧化铝凝胶辅助含有猪细小病毒的细胞培养液。佐剂对病毒和总蛋白的吸附量与佐剂浓度成正比。通过用1.2 M磷酸钾洗涤佐剂制剂，然后透析和浓缩洗涤液，实现了病毒和蛋白质从佐剂中以实质性、可重现的量解吸。十二烷基硫酸钠聚丙烯酰胺凝胶电泳无佐剂的病毒液，佐剂混合物的上清液，和解吸洗涤相应的佐剂颗粒显示蛋白质带型没有质的差异。通过血凝试验或采用单克隆抗猪细小病毒抗体的酶联免疫测定法定量解吸病毒。病毒解吸和随后的测定允许在体外估计佐剂猪细小病毒制剂中的病毒含量。该方法可用于估计灭活氢氧化铝佐剂兽用疫苗的抗原含量，并减少所需的体内检测范围。
Cell culture fluids containing porcine parvovirus were adjuvanted with varying concentrations of aluminum hydroxide gel. Adsorption of virus and total protein to adjuvant was proportional to adjuvant concentration. Desorption of virus and protein from the adjuvant in substantial, reproducible quantities was achieved by washing adjuvanted preparations with 1.2 M potassium phosphate, followed by dialysis and concentration of wash fluids. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis of unadjuvanted viral fluids, supernatants of adjuvanted mixtures, and desorptive washings of the corresponding adjuvant pellets revealed no qualitative differences in protein banding patterns. Desorbed virus was quantitated by a hemagglutination assay, or by an enzymelinked immunoassay employing a monoclonal anti-porcine parvovirus antibody. Virus desorption and subsequent assays permitted in vitro estimation of virus content in adjuvanted porcine parvovirus preparations. This approach may be useful in estimating the antigen content of inactivated aluminum hydroxide adjuvanted veterinary vaccines and reducing the extent of required in vivo testing.