Analysis of Cry1Ab toxin bioaccumulation in a food chain of Bt rice, an herbivore and a predator
Analysis of Cry1Ab toxin bioaccumulation in a food chain of Bt rice, an herbivore and a predator
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Bt 水稻、食草动物和捕食者食物链中 Cry1Ab 毒素的生物累积分析
DOI：
10.1007/s10646-008-0276-z
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发表时间：
2009-02-01
期刊：
影响因子：
2.7
通讯作者：
Shelton, Anthony M.
中科院分区：
文献类型：
--
作者：
Chen, Mao;Ye, Gong-yin;Shelton, Anthony M.
有机磷农药在不同营养级的非靶标生物体内积累，并产生意想不到的负面影响，因此有必要研究苏云金芽孢杆菌（Bacillusthuringiensis，Bt）杀虫毒素在Bt植物体内的生物转移和生物积累的可能性。利用ELISA方法研究了Cry1Ab毒素在Bt水稻（KMD 1和KMD 2）、靶标昆虫稻纵卷叶螟（Cnaphalocrocismedinalis）及其捕食性天敌水狼蛛（Piratasubpiraticus）食物链中的转移。Cry1Ab在C.medinalis和P.subpiraticus中均有检出。而在稻纵卷叶螟和拟海盗中检测到的Cry1Ab浓度并不随取食或捕食时间的增加而增加。对Cry1Ab与麦地那龙线虫（C.medinalisandP.subpiraticus）刷状缘膜囊泡结合的研究表明，P.subpiraticus中肠没有Cry1Ab结合受体，而麦地那龙线虫有。取食Bt稻螟的拟海盗的存活率和繁殖力与取食非Bt稻螟的拟海盗无显著差异。取食Bt水稻的稻纵卷叶螟的发育时间显著长于取食非Bt水稻的稻纵卷叶螟。然而，3年的田间试验表明，Bt水稻没有显着影响的密度。
Lessons from organophosphorus pesticides, which could be bioaccumulated in non-target organisms at different trophic levels and caused unexpected negative impacts, necessitate a study of the possibility of biotransfer and bioaccumulation ofBacillusthuringiensis(Bt) insecticidal toxin(s) expressed in Bt plants. Using ELISA, we evaluated the transfer of Cry1Ab toxin in a food chain of Bt rice (KMD1 and KMD2), the target insect,Cnaphalocrocismedinalis,and its predator,Piratasubpiraticus. Cry1Ab was detected inC.medinalisandP.subpiraticus. However, the concentration of Cry1Ab detected fromC.medinalisandP.subpiraticusdid not increase as feeding or preying time increased. A binding study of Cry1Ab to the brush border membrane vesicle ofC.medinalisandP.subpiraticusindicated thatP.subpiraticusdoes not have binding receptors in its midgut to Cry1Ab, whileC.medinalisdoes. Survivorship and fecundity ofP.subpiraticuspreying on Bt rice-fedC.medinaliswere not significantly different from those preying on non-Bt rice-fedC.medinalis. Developmental time ofP.subpiraticuswas significantly longer when it preyed on Bt rice-fedC.medinalisthan on non-Bt rice-fed prey. However, a 3-year field trial indicated that Bt rice did not significantly affect the density ofP.subpiraticus.