Detecting and minimizing glycosidase activities that can hydrolyze sugars from cell culture-produced glycoproteins
Detecting and minimizing glycosidase activities that can hydrolyze sugars from cell culture-produced glycoproteins
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DOI：
10.1385/mb:15:1:69
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发表时间：
2000-05-01
影响因子：
2.6
通讯作者：
Gramer, MJ
中科院分区：
文献类型：
--
作者：
Gramer, MJ
细胞培养产生的糖蛋白的异质性通常是由于糖蛋白寡糖的末端存在或不存在一些糖。生物工艺因素的变异性可能导致寡糖异质性的变异性（1）。尽管细胞内生物合成过程中的随机事件长期以来被认为是寡糖异质性的原因（2），但最近的数据表明，糖苷酶的细胞外降解也会导致寡糖异质性（3，4）。本章的目的是介绍糖苷酶降解的概念和后果，讨论评估糖苷酶降解是否对特定过程有意义的方法，并提供一些潜在的补救措施来减轻不良降解。
Heterogeneity of cell culture-produced glycoproteins often results from the presence or absence of a few sugars found on the tel minus of glycoprotein oligosaccharides. Variability in bioprocess factors can potentially lead to variability in this oligosaccharide heterogeneity (1). Although stochastic events in the intracellular biosynthetic process have long been recognized as a cause of oligosaccharide heterogeneity (2), more recent data has demonstrated that extracellular degradation by glycosidases call also contribute to oligosaccharide heterogeneity (3,4). The purpose of this chapter is to introduce the concept and consequence of glycosidase degradation, to discuss methods for evaluating whether glycosidase degradation is significant fur a particular process, and to provide some potential remedies to alleviate undesirable degradation.