Bonding mechanism and ultrastructural interfacial analysis of a single-step adhesive to dentin.
Bonding mechanism and ultrastructural interfacial analysis of a single-step adhesive to dentin.
复制标题
单步粘合剂与牙本质的粘合机制和超微结构界面分析。
DOI：
10.1016/s0109-5641(03)00017-4
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Pashley,DavidH
中科院分区：
文献类型：
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作者：
Ikemura,Kunio;Tay,FranklinR;Hironaka,Toshiya;Endo,Takeshi;Pashley,DavidH
目的研究商品化多合一粘接剂（Reactmer Bond）中的关键成分4-丙烯酰氧乙基偏苯三酸（4-AET）与牙本质磷灰石的相互作用，测定4-AET钙（4-AETCa）的溶解度，并观察该粘接剂粘接树脂-牙本质界面的超微结构。扁平的牛牙本质块用0.1wt%NaOCl处理10 min，或5.0wt%NaOCl处理10 min，或30 min。使用衰减全反射（FT-IR/ATR）的傅立叶变换红外分析在这四个基板上进行处理前后与4-AET混合物（4-AET/HEMA：40/60wt%）。结果4-AETCa在水、甲基丙烯酸2-羟乙酯、水溶液中的溶解度分别为0.001、0.01、0.0（HEMA; 100wt%）和HEMA/水（70/30wt%）在2 min后的摩尔浓度分别为0.023、0.003和0.014mol/l（新条带：νC O：1580和1413 cm−1）在所有牙本质样本上形成后，应用4-AET，有或没有NaOCl预处理。透射电镜观察显示，在1 μm厚的混合层中，有少量的磷灰石晶粒被一层电子密度较低的材料包裹，提示4-AET中游离的羧基可能与部分脱矿混合层中磷灰石晶粒中的Ca ~（2+）相互作用，形成不溶性的钙盐（4-AETCa），这可能有助于该树脂系统与牙本质的结合。
Objectivesto characterize the interaction of 4-acryloxyethyltrimellitic acid (4-AET), a key ingredient in a commercial all-in-one adhesive (Reactmer Bond) with dentin apatite, to measure the solubility of 4-AET calcium (4-AETCa), and to examine the ultrastructure of the resin–dentin interface bonded by this adhesive.Methodssolubility of synthesized 4-AETCa in several solutions was measured. Flattened bovine dentin blocks were treated with 0.1wt% NaOCl for 10 min, or 5.0wt% NaOCl for 10 min, or 30 min. Fourier transform infrared analysis using attenuated total reflection (FT-IR/ATR) was performed on these four substrates before and after treatment with a 4-AET mixture (4-AET/HEMA: 40/60wt%). Undemineralized, unstained sections of human sound dentin that were bonded with the same comonomers were examined by transmission electron microscopy (TEM).Resultsthe respective solubilities of the 4-AETCa in water, 2-hydroxyethyl methacrylate (HEMA; 100wt%) and HEMA/water (70/30wt%) were 0.023, 0.003 and 0.014 mol/l after 2 min. The IR analysis indicated that Ca-carboxylate (new bands: νCO:1580 and 1413 cm−1) was formed on all dentin specimens following application of 4-AET, with or without NaOCl pretreatment. TEM showed a 1 μm thick hybrid layer with some remnant apatite crystallites that were encapsulated by a layer of less electron-dense material.Significancethe results suggest that ionized carboxyl groups in 4-AET may interact with Ca2+from apatite crystallites within the partially demineralized hybrid layer to form an insoluble calcium salt (4-AETCa) that may aid in bonding this resin system to dentin.