Species-dependent changes in stomatal sensitivity to abscisic acid mediated by external pH
Species-dependent changes in stomatal sensitivity to abscisic acid mediated by external pH
复制标题
DOI：
10.1093/jxb/erj057
复制
发表时间：
2006-02-01
影响因子：
6.9
通讯作者：
Stikic, R
中科院分区：
文献类型：
--
作者：
Prokic, L;Jovanovic, Z;Stikic, R
在鸭跖草和拟南芥的表皮条中检查了 pH 变化和/或脱落酸 (ABA) 对气孔孔径的直接影响。气孔最初在 pH 7 或 pH 5 下打开。使用视频显微镜监测由外部 pH 和/或 ABA（10 μM 或 10 nM）变化引起的气孔关闭，并使用图像分析软件根据气孔面积的变化进行量化。孔径面积的测量能够可靠地量化气孔反应，特别是气孔面积的微小变化。两种植物都表现出对 ABA 的双相关闭反应：气孔快速关闭的初始阶段，随后是第二个更长的阶段，在此期间气孔关闭以较慢的速度进行。还观察到气孔对 ABA 敏感性的变化。对 C.Communis 和 A.thaliana 之间的这些影响进行比较表明，气孔对 ABA 的这种差异敏感性是物种依赖性的，并且还取决于细胞外环境的 pH 值。
The direct effects of pH changes and/or abscisic acid (ABA) on stomatal aperture were examined in epidermal strips of Commelina communis L. and Arabidopsis thaliana. Stomata were initially opened at pH 7 or pH 5. The stomatal closure induced by changes in external pH and/or ABA (10 mu M or 10 nM) was monitored using video microscopy and quantified in terms of changes in stomatal area using image analysis software. Measurements of aperture area enabled stomatal responses and, in particular, small changes in stomatal area to be quantified reliably. Both plant species exhibited a biphasic closure response to ABA: an initial phase of rapid stomatal closure, followed by a second, more prolonged, phase during which stomata closure proceeded at a slower rate. Changes in stomatal sensitivity to ABA were also observed. Comparison of these effects between C. communis and A. thaliana demonstrate that this differential sensitivity of stomata to ABA is species-dependent, as well as being dependent on the pH of the extracellular environment.