Developing Rice with High Yield under Phosphorus Deficiency: Pup1 Sequence to Application
Developing Rice with High Yield under Phosphorus Deficiency: Pup1 Sequence to Application
复制标题
DOI：
10.1104/pp.111.175471
复制
发表时间：
2011-07-01
期刊：
影响因子：
7.4
通讯作者：
Heuer, Sigrid
中科院分区：
文献类型：
--
作者：
Chin, Joong Hyoun;Gamuyao, Rico;Heuer, Sigrid
磷吸收主效QTL(Pup1)是决定土壤耐缺磷能力的主效QTL，是目前水稻耐低磷品种选育中最有前景的QTL之一。为了便于将Pup1定向导入到不耐受品种中，利用供体品种Kasalath中Pup1区域预测的基因模型来开发基于基因的分子标记，这些标记均匀分布在精细定位的278kb的QTL区域上。为了验证基因模型和优化标记，进行了基因表达分析和部分等位基因测序。这些标记在80多个不同的水稻材料上进行了检测，发现有三个主要群体具有不同的Pup1等位基因组成。在所分析的部分基因座(部分Pup1；组II)上，Pup1耐性(I组)和不耐受(III组)Pup1等位基因与Kasalath等位基因(PUP1、II)不同。一项种质调查进一步证实了早先的数据，即Pup1在灌溉水稻品种中大部分缺失，但在适应干旱易发环境的品种和育种系中保持保守。定义了一组核心的Pup1标记，并鉴定了适合于高通量基因分型的单核苷酸多态标记开发的序列多态。通过标记辅助回交的方法，将Pup1导入两个灌溉水稻品种和三个印尼旱地品种。首先，对导入系的表型评价表明，Pup1在不同的遗传背景和环境中是有效的，在大田条件下具有显着提高粮食产量的潜力。
The major quantitative trait locus (QTL) Phosphorus uptake1 (Pup1) confers tolerance of phosphorus deficiency in soil and is currently one of the most promising QTLs for the development of tolerant rice (Oryza sativa) varieties. To facilitate targeted introgression of Pup1 into intolerant varieties, the gene models predicted in the Pup1 region in the donor variety Kasalath were used to develop gene-based molecular markers that are evenly distributed over the fine-mapped 278-kb QTL region. To validate the gene models and optimize the markers, gene expression analyses and partial allelic sequencing were conducted. The markers were tested in more than 80 diverse rice accessions revealing three main groups with different Pup1 allele constitution. Accessions with tolerant (group I) and intolerant (group III) Pup1 alleles were distinguished from genotypes with Kasalath alleles at some of the analyzed loci (partial Pup1; group II). A germplasm survey additionally confirmed earlier data showing that Pup1 is largely absent from irrigated rice varieties but conserved in varieties and breeding lines adapted to drought-prone environments. A core set of Pup1 markers has been defined, and sequence polymorphisms suitable for single-nucleotide polymorphism marker development for high-throughput genotyping were identified. Following a marker-assisted backcrossing approach, Pup1 was introgressed into two irrigated rice varieties and three Indonesian upland varieties. First phenotypic evaluations of the introgression lines suggest that Pup1 is effective in different genetic backgrounds and environments and that it has the potential to significantly enhance grain yield under field conditions.