Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum
Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum
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短茄和马铃薯原生质体融合体细胞杂种的生育力
DOI：
10.1007/bf00262507
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发表时间：
2004
影响因子：
5.4
通讯作者：
J. Helgeson
中科院分区：
文献类型：
--
作者：
M. Ehlenfeldt;J. Helgeson
短茄属 (Solanum brevidens) (2x) 和 Solanum brevidens 之间的两组体细胞杂交种。对马铃薯（2x 和 4x）的雄性生育力、减数分裂规律和雌性生育力进行评估。体细胞杂种是来自2x+2x融合的四倍体和来自2x+4x融合的六倍体。四倍体花粉染色度范围为 0 至 83%，六倍体花粉染色度范围为 0 至 23%。与六倍体相比，四倍体具有更规则的减数分裂、更低水平的微花粉和更少的不相关染色体。然而，除了自交水平较低之外，两组杂种的花粉在授粉方面均无效。作为雌性的四倍体与 2x 和 4x 测试物种的杂交效果较差，并且自交水平较低。六倍体融合杂种与 2x 测试物种的杂交也很差，并且自交仅达到有限程度；然而，它们与 4x 测试者的杂交效果很好。与 S 杂交结籽。马铃薯组Andigena，和S。马铃薯组马铃薯品种“Kathadin”和“Norland”的平均每个果实分别有 16.7、15.6 和 28.6 个种子。这些杂交的后代具有 5 倍或接近 5 倍倍性水平。结果表明，S. 体细胞融合杂种可以获得合理水平的雌性生育力。布雷维登桑S.马铃薯。
Two sets of somatic hybrids betweenSolanum brevidens(2x) andS. tuberosum(2x and 4x) were evaluated for male fertility, meiotic regularity and female fertility. The somatic hybrids were tetraploids from 2x + 2x fusions and hexaploids from 2x + 4x fusions. Pollen stainability ranged from 0 to 83% in tetraploids and from 0 to 23% in hexaploids. The tetraploids had more regular meiosis, lower levels of micropollen and fewer unassociated chromosomes than hexaploids. However, except for a low level of selfing, the pollen of both sets of hybrids was ineffective in pollinations. The tetraploids, as females, crossed poorly with 2x and 4x tester species and selfed only at low levels. The hexaploid fusion hybrids also crossed poorly with the 2x tester species and selfed only to a limited degree; however, they crossed well with 4x testers. Seed set in crosses withS. tuberosumGroup Andigena, andS. tuberosumGroup Tuberosum cultivars ‘Kathadin’ and ‘Norland’ averaged 16.7, 15.6 and 28.6 seeds per fruit, respectively. Progeny from these crosses had 5x or nearly 5x ploidy levels. The results indicate that reasonable levels of female fertility can be obtained in somatic fusion hybrids ofS. brevidensandS. tuberosum.