Transcriptome analysis of bud burst in sessile oak (Quercus petraea)
Transcriptome analysis of bud burst in sessile oak (Quercus petraea)
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DOI：
10.1111/j.1469-8137.2006.01721.x
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发表时间：
2006-01-01
期刊：
影响因子：
9.4
通讯作者：
Kremer, A
中科院分区：
文献类型：
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作者：
Derory, J;Léger, P;Kremer, A
在无梗花栎（Quercus petraea）芽萌发过程中，对顶芽中数百种转录本的表达模式进行了监测，以鉴定在芽发育的休眠期和活跃期之间差异表达的基因。采用不同的转录组技术，包括构建抑制性差减杂交（SSH）文库以及使用宏阵列和实时逆转录酶聚合酶链反应（RT - PCR）监测基因表达，对芽萌发进行剖析，尤其着重于该过程的起始阶段。我们从芽萌发的六个发育阶段获得了801个表达序列标签（ESTs）。宏阵列实验显示，在该过程中共有233种独特的转录本表现出差异表达，其中65%被赋予了假定功能。细胞拯救/防御、代谢、蛋白质合成、细胞周期和转录相关的转录本属于受调控程度最高的基因。宏阵列和实时RT - PCR表明，一些基因在休眠芽和膨大芽之间表现出相反的表达，例如转录因子DAG2（影响萌发的Dof蛋白2）的一个假定同源物，此前据报道该基因参与拟南芥种子萌发的控制。这些差异表达的基因是树木芽萌发信号通路的相关候选基因。
circle Expression patterns of hundreds of transcripts in apical buds were monitored during bud flushing in sessile oak (Quercus petraea), in order to identify genes differentially expressed between the quiescent and active stage of bud development.circle Different transcriptomic techniques combining the construction of suppression subtractive hybridization (SSH) libraries and the monitoring of gene expression using macroarray and real-time reverse transcriptase polymerase chain reaction (RT-PCR) were performed to dissect bud burst, with a special emphasis on the onset of the process.circle We generated 801 expressed sequence tags (ESTs) derived from six developmental stages of bud burst. Macroarray experiment revealed a total of 233 unique transcripts exhibiting differential expression during the process, and a putative function was assigned to 65% of them. Cell rescue/defense-, metabolism-, protein synthesis-, cell cycle- and transcription-related transcripts were among the most regulated genes. Macroarray and real-time RT-PCR showed that several genes exhibited contrasted expressions between quiescent and swelling buds, such as a putative homologue of the transcription factor DAG2 (Dof Affecting Germination 2), previously reported to be involved in the control of seed germination in Arabidopsis thaliana.circle These differentially expressed genes constitute relevant candidates for signaling pathway of bud burst in trees.