Who Is Metabolizing What? Discovering Novel Biomolecules in the Microbiome and the Organisms Who Make Them
Who Is Metabolizing What? Discovering Novel Biomolecules in the Microbiome and the Organisms Who Make Them
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DOI：
10.3389/fcimb.2020.00388
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发表时间：
2020-07-31
影响因子：
5.7
通讯作者：
Metz, Thomas O.
中科院分区：
文献类型：
--
作者：
Couvillion, Sneha P.;Agrawal, Neha;Metz, Thomas O.
尽管微生物组研究领域在绘制各种环境和生物体中的微生物群落组成方面取得了巨大进步，但解释微生物类群（已知和未知）对宿主的表型影响及其特定功能仍然是主要挑战。迫切需要的是能够将酶和小分子的特定功能分配给群落中的特定分类群或分类群。这些知识对于推进基于微生物或代谢物的靶向调节的个性化治疗至关重要，这些微生物或代谢物具有可预测的结果，使人类宿主受益。这篇观点文章主张结合使用无标准代谢组学和基于活性的蛋白质分析策略，通过识别新型生物分子并将其生产归因于特定的微生物类群，来解决微生物组研究中功能知识的这一差距。
Even as the field of microbiome research has made huge strides in mapping microbial community composition in a variety of environments and organisms, explaining the phenotypic influences on the host by microbial taxa-both known and unknown-and their specific functions still remain major challenges. A pressing need is the ability to assign specific functions in terms of enzymes and small molecules to specific taxa or groups of taxa in the community. This knowledge will be crucial for advancing personalized therapies based on the targeted modulation of microbes or metabolites that have predictable outcomes to benefit the human host. This perspective article advocates for the combined use of standards-free metabolomics and activity-based protein profiling strategies to address this gap in functional knowledge in microbiome research via the identification of novel biomolecules and the attribution of their production to specific microbial taxa.