Reduction in the colonization of Staphylococcus aureus on the skin surface under calcium-/magnesium-depleted conditions. Letters in Applied Microbiology.
Reduction in the colonization of Staphylococcus aureus on the skin surface under calcium-/magnesium-depleted conditions. Letters in Applied Microbiology.
复制标题
在钙/镁耗尽的条件下，减少金黄色葡萄球菌在皮肤表面的定植。
DOI：
10.1111/lam.13037
复制
发表时间：
2018
影响因子：
2.4
通讯作者：
Tanaka A.
中科院分区：
文献类型：
--
作者：
Amagai Y;Makita Y;Takai M;Muko R;Matsuda H;Tanaka A.
金黄色葡萄球菌的过度扩张与包括特应性皮炎（AD）在内的多种皮肤病有关。最近，我们已经证明，用含有少量二价金属离子的超纯软水清洗皮肤可以改善特应性受试者的皮肤状况。本研究探讨了钙、镁对S.在体外和体内的金黄色葡萄球菌。培养基中钙和镁的缺乏显著抑制了S.金色生长当S.将金黄色葡萄球菌（用含钙/镁的水稀释至中等硬度的水（83·0 mg l− 1，以CaCO 3计）或含少量钙/镁的水稀释）涂敷到Hos：HR-1小鼠胶带剥离的皮肤上，S.皮肤上没有这些矿物质的金色水被抑制。这些结果表明，钙和镁的耗尽废除S的增殖。金黄色葡萄球菌不仅在培养体系中，而且在小鼠皮肤表面。自从S.众所周知，皮肤上的金黄色葡萄球菌会加剧AD症状，使用含有较少钙和镁的超纯软水可能会通过抑制金黄色葡萄球菌来改善皮肤状况。金黄色葡萄球菌在皮肤问题患者皮肤上的生长。研究的意义和影响本研究通过使用体外培养系统和小鼠皮肤上的体内实验来证明钙和镁对于金黄色葡萄球菌的定植和生长的重要性。我们的研究结果表明，这些金属离子的去除可能有利于保护皮肤免受S。金黄色。因此，使用不含金属离子的超纯软水可以通过保护皮肤免受S.金色殖民
Excessive expansion ofStaphylococcus aureusis associated with several skin diseases, including atopic dermatitis (AD). Recently, we have demonstrated that washing skins with ultra‐pure soft water containing little bivalent metal ions improved skin conditions of atopic subjects. In this study, we investigated the roles of calcium or magnesium on the proliferation ofS. aureusbothin vitroandin vivo. Depletion of calcium and magnesium in the culture medium significantly suppressed the expansion ofS. aureusgrowth. WhenS. aureus, diluted with water containing calcium/magnesium at the concentration of medium‐hard water (83·0 mg l−1as CaCO3) or the one that contains little calcium/magnesium, was applied onto the tape‐stripped skin of Hos:HR‐1 mice, growth ofS. aureusin water without those minerals on the skin was suppressed. These results suggest that depletion of both calcium and magnesium abrogate the proliferation ofS. aureusnot only in the culture system but also on the skin surface of mice. Since colonization ofS. aureuson the skin is well‐known to exacerbate AD symptoms, usage of ultra‐pure soft water containing less calcium and magnesium may improve the skin condition through the suppression ofS. aureusgrowth on the skin of patients with skin problems.Significance and Impact of the StudyThis study demonstrates the importance of calcium and magnesium for the colonization and growth ofStaphylococcus aureusby using bothin vitroculture systems andin vivoexperiments on the murine skin. Our results indicate that the removal of these metal ions is probably beneficial for protecting the skin fromS. aureus. Thus, using ultra‐pure soft water without metal ions may improve the skin condition of patients with skin problems through the protection fromS. aureuscolonization.