Spatial comparison between wall shear stress measures and porcine arterial endothelial permeability
Spatial comparison between wall shear stress measures and porcine arterial endothelial permeability
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DOI：
10.1152/ajpheart.00897.2003
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发表时间：
2004-05-01
影响因子：
4.8
通讯作者：
Friedman, MH
中科院分区：
文献类型：
--
作者：
Himburg, HA;Grzybowski, DM;Friedman, MH
更好地了解血流动力学因素如何影响血管内皮的完整性和功能是必要的，以更充分地了解动脉粥样硬化是如何启动和促进的。提出了一种新的技术来评估流体动力学变量和内皮细胞对大分子的渗透性之间的关系。将完全麻醉的家猪静脉内注射白蛋白标记物Evans蓝染料，使其循环90 min。动物安乐死后，制作腹主动脉及其髂动脉分支的硅胶管型。脉动流计算随后在计算区域从铸件。计算出的时间依赖性壁剪切应力在髂外分支的分布直接进行了比较，在一个逐点的基础上，在相同的动脉中的伊文思蓝染料的体内摄取的空间变化。结果表明，在正常范围内，随着时间平均剪切应力的增加，体内内皮细胞对白蛋白的渗透性降低。此外，内皮渗透性随振荡剪切指数略有增加。
A better understanding of how hemodynamic factors affect the integrity and function of the vascular endothelium is necessary to appreciate more fully how atherosclerosis is initiated and promoted. A novel technique is presented to assess the relation between fluid dynamic variables and the permeability of the endothelium to macromolecules. Fully anesthetized, domestic swine were intravenously injected with the albumin marker Evans blue dye, which was allowed to circulate for 90 min. After the animals were euthanized, silicone casts were made of the abdominal aorta and its iliac branches. Pulsatile flow calculations were subsequently made in computational regions derived from the casts. The distribution of the calculated time-dependent wall shear stress in the external iliac branches was directly compared on a point-by-point basis with the spatially varying in vivo uptake of Evans blue dye in the same arteries. The results indicate that in vivo endothelial permeability to albumin decreases with increasing time-average shear stress over the normal range. Additionally, endothelial permeability increases slightly with oscillatory shear index.