Metabolic engineering to produce sesquiterpenes in yeast
Metabolic engineering to produce sesquiterpenes in yeast
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DOI：
10.1021/ol034231x
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发表时间：
2003-05-15
期刊：
影响因子：
5.2
通讯作者：
Matsuda, SPT
中科院分区：
文献类型：
--
作者：
Jackson, BE;Hart-Wells, EA;Matsuda, SPT
[附图]这里展示的是一种代谢工程酵母菌株，它能产生倍半萜。在一株天然酵母中表达的表松醇合成酶将内源焦磷酸法尼基转化为90mug/L的表柏醇。对该系统进行了基因改造，将外源萜烯产量提高到370mug/L。在Upc2-1交配型a背景中，通过过度表达截短的3-羟基-3-甲基戊二酰辅酶A(HMG-CoA)还原酶获得了最好的产量。这个系统允许足够的产量来表征新的倍半萜合成酶基因。
[GRAPHICS]Presented here is a metabolically engineered yeast strain that produces sesquiterpenes. Epi-cedrol synthase expressed in a native yeast strain converted endogenous farnesyl pyrophosphate to 90 mug/L epi-cedrol. This system was genetically modified to increase foreign terpene yields to 370 mug/L. The best yields were obtained by overexpressing a truncated 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in a upc2-1 mating type a background. This system allows sufficient production to characterize novel sesquiterpene synthase genes.