Liver Hydrolysate Assists in the Recovery From Physical Fatigue in a Mouse Model
Liver Hydrolysate Assists in the Recovery From Physical Fatigue in a Mouse Model
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DOI：
10.1254/jphs.13084fp
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发表时间：
2013-12-01
影响因子：
3.5
通讯作者：
Tan-No, Koichi
中科院分区：
文献类型：
--
作者：
Nakagawasai, Osamu;Yamada, Kotaro;Tan-No, Koichi
据报道，肝水解物（LH）可增强肝功能。然而，LH对身体疲劳的影响尚不清楚。本研究的目的是研究LH对强迫行走后运动活动和能量代谢如5 ′-AMP-活化蛋白激酶（AMPK）、糖原含量和血乳酸的变化的影响。自发活动，AMPK磷酸化，肝和比目鱼肌中的糖原含量，以及血乳酸测定LH治疗前和/或后强迫行走。强迫步行3 h后，自发活动明显减少。两次LH给药（30或100 mg/kg）显著增加了自发活动，而在强迫行走之前或之后的单次给药未显示出任何特异性作用。给予LH两次激活肝脏和比目鱼肌中的AMPK。糖原水平显着下降，在肝脏和比目鱼肌强迫行走后，而血乳酸水平显着增加。相反，给予LH两次增加肌糖原和降低血乳酸。这些结果表明，LH产生抗疲劳作用，这种作用似乎涉及通过激活AMPK有效利用糖原。
It is reported that liver hydrolysate (LH) enhances liver function. However, the effects of LH on physical fatigue are unknown. The aim of this study was to investigate the effect of LH on alterations in locomotor activity and energy metabolism such as 5'-AMP-activated protein kinase (AMPK), glycogen content, and blood lactic acid, after forced walking Adult male ddY mice were used. Locomotor activity, AMPK phosphorylation, and glycogen content in the liver and soleus muscle, as well as blood lactic acid were determined following LH treatment before and/or after forced walking. The locomotor activity significantly decreased after forced walking for 3 h. Two administrations of LH (30 or 100 mg/kg) significantly increased the locomotor activity, while a single administration either before or after forced walking did not show any specific effect. Administering LH twice activated AMPK in the liver and soleus muscle. Glycogen levels significantly decreased in both the liver and soleus muscle after forced walking, whereas the blood lactate level significantly increased. In contrast, administering LH twice increased muscle glycogen and decreased blood lactic acid. These findings indicate that LH produced an anti-fatigue effect and that this effect appears to involve the efficient glycogen utilization through activation of AMPK.