No extinction of tyrosine aminotransferase inducibility in rat hepatoma-human fibroblast hybrids containing the human X chromosome.
No extinction of tyrosine aminotransferase inducibility in rat hepatoma-human fibroblast hybrids containing the human X chromosome.
复制标题
含有人 X 染色体的大鼠肝癌-人成纤维细胞杂交体中酪氨酸转氨酶诱导能力没有消失。
DOI：
10.1159/000131587
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
M. Weiss
中科院分区：
文献类型：
--
作者：
C. Sellem;D. Cassio;M. Weiss
几年前，CROCI et al.（1973）报道了人X染色体携带一个调节基因，该基因负责大鼠肝癌细胞酪氨酸氨基转移酶（达特; EC 2.6. 1.5）。我们试图证实中国仓鼠的这一分配（韦斯等人，1975）和大鼠（DESCHATRI m等，1979），但不能重现CROCI等的观察结果。因此，我们不得不取消他们最初的实验，即研究大鼠肝癌细胞与二倍体人WI 38成纤维细胞融合形成的杂交体中达特的诱导。在检测的14个独立的杂交克隆中，没有一个显示酶的诱导物的消失，而人形式的葡萄糖-6-磷酸脱氢酶（G6 PD; EC 1.1. 1.49），一个X连锁标记，存在于所有的。
Several years ago, CROCI et al.(1973) reported that the human X chromosome carries a regulatory gene responsible for the extinction of glucocorticoid hormone induc ibility of rat hepatoma cell tyrosine amino transferase (TAT; EC 2.6. 1.5). We at tempted to confirm this assignment for the Chinese hamster (WEISS et al., 1975) and the rat (DESCHATRI m et al., 1979), but were unable to reproduce the observations of CROCI et al. We have therefore under taken to repeal their original experiments, that is, to study the inducibility of TAT in hybrids formed by fusing rat hepatoma cells with diploid human WI38 fibroblasts. Among the 14 independent hybrid clones examined, none showed extinction of induc ibility of the enzyme, while the human form of glucose-6-phosphate dehydrogenase (G6PD; EC 1.1. 1.49), an X-linked marker, was present in all of them.